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We narrowed to 902 results for: Ide

Showing: 501 - 520 of 902 results

  1. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... DNA sequence to encode your peptide of interest and produce that peptide in your organism of choice. ...as easy as it sounds. Even for relatively short peptides, there can be many possible ways to encode them... the native organism.” This is possibly a better idea and has been used successfully in the past (Angov...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...PubMed Central PMCID: PMC4794256. This review provides a great overview of codon usage bias 8. Tuller...

  2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...Fluorescent and bioluminescent reporter genes are widely used to study gene expression in cells. But these... by tissue. Ultrasound, on the other hand, is a widely available and inexpensive imaging modality with...high spatial and temporal resolution, making it ideal for visualizing gene expression in vivo.  But what... be easily expressed heterologously by bacteria inside the body. So we screened gas vesicles gene clusters...origin of replication (p15A) worked best under a wide range of inducer concentrations (0.1-1% L-arabinose...bARGSer-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical B-mode...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...have accumulated more than 300,000 individual nucleotide sequences. More than half of these sequences ...assembly algorithms meant that we already had a clear idea of the necessary steps to perform:      ...entire package to run. The small, portable system provided by a container is sequestered from other programs...container because of the reproducibility containers provide and for convenient compatibility with our internal...complete pipeline however, required more than just identifying criteria that we needed. Prior to using Nextflow...that the desired data was available so we felt confident we were using the input and output to link the...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...600 Cas9 orthologs and found that they could be divided into two groups: longer orthologs approximately...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates... CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature 566:218–223 . https://doi.org...2014) Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell 156:935–949. https://doi.org...doi.org/10.1038/s41467-018-08224-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur... Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...

  5. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...might not localize to the right places, which can provide misleading information on where exactly a structure... via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence...sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm of... the expression of nearly any gene via specific guide RNA (gRNA) sequences. But these approaches rely ... viral aliquots from our plasmid collection to provide ready-to-use viral preps. Here are some of the ...

  6. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...others can provide you with the highest form of flattery - imitation! Use the MIQE guidelines (Bustin, ...primer and be at least 25 bp away from each primer, ideally located close to either the forward or the reverse...across the plate is fine; a 5% positive error on one side and a 5% negative error on the other will introduce...triplicates in multiple configurations can help you identify pipetting or evaporation errors. And if time allows...look at their 551 page e-book on applying MIQE guidelines! With these kinds of resources, you will soon...Shipley GL; Vandesompele J; Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative...

  7. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...and find our guides, plasmid kits and other resources for microbial research. Kits, guides, plasmids, ...biology, gene cloning and expression, as well as guide pages for Synthetic Biology and Bacterial Expression... our Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques...Expression and Insert Species filters on the right-hand side of the search page to narrow your keyword search...

  8. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Twitter for the official launch!  Videos Look out for our next protocol video, “Western blots,” coming soon...also be building our video library all summer long, with additions to our other video series.   And if you...as excited as we are! Come join us in our blog, video, and social media endeavors - in between some much-deserved...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...frequency three orders of magnitude higher than identical targeting vectors delivered through transfection...AAV effect remains unclear, it is likely not a coincidence that the AAV genome is composed of single stranded... selection, individual gene edited clones are identified by PCR. Then, if necessary for subsequent experiments...disadvantages to the AAV-based approach that must be considered when deciding whether to use AAV or CRISPR for...for the generation of knockins, it is not always ideal. Because of these limitations, the AAV approach ...variety of cell types, now you know that AAVs can provide a powerful boost to genome editing experiments....

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...for DMD treatment. Clinical trials have used oligonucleotide exon skipping (OEN) to remove mutated exons... dystrophin transcript. Unfortunately, the oligonucleotides only modestly improve muscle function, and...Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges, researchers...rigorously examined over a long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be ...Covello G, Denti MA. Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases... Resources on Addgene.org Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find...

  11. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family of small...or DNA. Now they have developed this further to provide multiplexed detection, higher sensitivity, and ...of DNA Elements (ENCODE), a consortium aimed to identify functional elements in the human genome, these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

  12. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the...plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...world... Where did 500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts... improve the repository. We love being able to provide a useful (and growing) resource for our favorite...

  13. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...Plasmids 101 videos and needed a break so they're hanging out with Aavery in this Zoom video background....background. Want the purple bacteria video background with mascots Blugene and Aavery? Download the 720p version...our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral preps! Download...DNA background. Or get the same background as a video background! (720 px or 1080 px).         Do you...

  14. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...library screening, read our recent blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning...v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has...of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly...Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening." Nature methods ...Resources on the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm, your experimental...tools, where modification of cellular activity outside of your experiment site or circuit can occur. Promoters... promoters  This type of promoter allows you to decide when to turn on, or turn off, gene expression by...promoters to begin testing, you’ll next need to decide how much virus to put into your system, which is...injection volume.  There are a few factors to consider. First, the titer required is inversely related... posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck, and...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...when enhancers were active or silent, they could identify the target genes of previously uncharacterized...has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and the epigenetic...this fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part...CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair... the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment with... Resources on Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic...

  17. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ... coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful teams...this series). Members carry on back-channel or side conversations within the team. We are not talking...contribute. Members periodically break, go exploring outside the team, and bring information back. No team ...colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead of emailing.    ...volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc...

  18. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...requesting and ordering. This split allows us to provide researchers with increased choice and flexibility... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested...testing, and viral genomic sequencing to confirm the identity of the packaged DNA and serotype. Please note ... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...1–2 days (USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan...

  19. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...Scientific Disciplines on Twitter. Twitter users were identified as scientists if they had a scientific title ...scientists within their fields than scientists outside their fields Figure 6: Follower Network of Scientists...follow scientists within their field rather than outside their field. Lower: Follower networks of subgroups... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...

  20. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ... a very different schedule. Fortunately, I was provided with a detailed training schedule that helped ...it relates to other teams at the company. This provides new (and old) employees with information on how...possible to work the remainder of the three hours (outside of the core hours) at a time that works for you...transition into a traditional work environment was aided by the efforts being put in by Addgene to ensure...blog.      More resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for...
Showing: 501 - 520 of 902 results