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Showing: 501 - 520 of 895 results

  1. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...and find our guides, plasmid kits and other resources for microbial research. Kits, guides, plasmids, ...biology, gene cloning and expression, as well as guide pages for Synthetic Biology and Bacterial Expression... our Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques...Expression and Insert Species filters on the right-hand side of the search page to narrow your keyword search...

  2. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Twitter for the official launch!  Videos Look out for our next protocol video, “Western blots,” coming soon...also be building our video library all summer long, with additions to our other video series.   And if you...as excited as we are! Come join us in our blog, video, and social media endeavors - in between some much-deserved...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...frequency three orders of magnitude higher than identical targeting vectors delivered through transfection...AAV effect remains unclear, it is likely not a coincidence that the AAV genome is composed of single stranded... selection, individual gene edited clones are identified by PCR. Then, if necessary for subsequent experiments...disadvantages to the AAV-based approach that must be considered when deciding whether to use AAV or CRISPR for...for the generation of knockins, it is not always ideal. Because of these limitations, the AAV approach ...variety of cell types, now you know that AAVs can provide a powerful boost to genome editing experiments....

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...for DMD treatment. Clinical trials have used oligonucleotide exon skipping (OEN) to remove mutated exons... dystrophin transcript. Unfortunately, the oligonucleotides only modestly improve muscle function, and...Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges, researchers...rigorously examined over a long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be ...Covello G, Denti MA. Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases... Resources on Addgene.org Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find...

  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family of small...or DNA. Now they have developed this further to provide multiplexed detection, higher sensitivity, and ...of DNA Elements (ENCODE), a consortium aimed to identify functional elements in the human genome, these...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...

  6. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the...plasmid shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. ...world... Where did 500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts... improve the repository. We love being able to provide a useful (and growing) resource for our favorite...

  7. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...Plasmids 101 videos and needed a break so they're hanging out with Aavery in this Zoom video background....background. Want the purple bacteria video background with mascots Blugene and Aavery? Download the 720p version...our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral preps! Download...DNA background. Or get the same background as a video background! (720 px or 1080 px).         Do you...

  8. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...library screening, read our recent blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning...v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has...of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly...Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening." Nature methods ...Resources on the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...there are up to thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition... the second number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure...AAVrg has not been thoroughly studied in contexts outside the nervous system to confirm its cellular receptors...2008). Adeno-Associated Virus (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1,...., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-...Capsids Resources on Addgene.org AAV Data Hub AAV Guide AAV Packaged On Request Browse all in-stock AAV ...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...when enhancers were active or silent, they could identify the target genes of previously uncharacterized...has made targeting much easier! With the help of guide RNAs, fusions between Cas proteins and the epigenetic...this fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part...CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair... the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment with... Resources on Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic...

  11. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm, your experimental...tools, where modification of cellular activity outside of your experiment site or circuit can occur. Promoters... promoters  This type of promoter allows you to decide when to turn on, or turn off, gene expression by...promoters to begin testing, you’ll next need to decide how much virus to put into your system, which is...injection volume.  There are a few factors to consider. First, the titer required is inversely related... posts in our Viral Vectors 101 series can help guide you with these, so keep reading! Good luck, and...

  12. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ... coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful teams...this series). Members carry on back-channel or side conversations within the team. We are not talking...contribute. Members periodically break, go exploring outside the team, and bring information back. No team ...colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead of emailing.    ...volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc...

  13. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...requesting and ordering. This split allows us to provide researchers with increased choice and flexibility... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested...testing, and viral genomic sequencing to confirm the identity of the packaged DNA and serotype. Please note ... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...1–2 days (USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan...

  14. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ... a very different schedule. Fortunately, I was provided with a detailed training schedule that helped ...it relates to other teams at the company. This provides new (and old) employees with information on how...possible to work the remainder of the three hours (outside of the core hours) at a time that works for you...transition into a traditional work environment was aided by the efforts being put in by Addgene to ensure...blog.      More resources on the Addgene blog A Guide to Getting Started in Undergrad Research Tips for...

  15. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...Scientific Disciplines on Twitter. Twitter users were identified as scientists if they had a scientific title ...scientists within their fields than scientists outside their fields Figure 6: Follower Network of Scientists...follow scientists within their field rather than outside their field. Lower: Follower networks of subgroups... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...

  16. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...certainly hope not! One of our core values here is to provide unmatched customer support, as this is vital to...exact problem, and it’s called “self-service.” The idea here is to build features that allow users to help...general question or concern about the answers we provide, within the widget you also have the option to ... we’ll be continually improving the support we provide based on your valuable feedback. This is Addgene...first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid...

  17. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014 Johnson & Johnson’...up to the discovery of the mechanism of dual-RNA-guided DNA cleavage in adaptive bacterial immunity and...Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven...thanked Martin Jinek and Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their... Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

  18. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Visit our viral vectors guide Find our chemogenetics and optogenetics science guides ...Chemogenetic approaches excel at broad tissue or system-wide targeting, as the ligand can reach target cells ...optogenetics. If broad control of whole circuits or tissue-wide systems is necessary, use chemogenetics. Note that...approaches suffer from unique sets of off-target side-effects that should be carefully controlled for ...

  19. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ... mapping where a protein binds genome-wide, and has been widely applied in many model organisms and cell... Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the... degree of signal to noise is desired. It also provides an excellent additional measure of DNA binding...technique will quickly become a standard method alongside ChIP. Many thanks to our guest blogger, Matthew...

  20. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process you... of small molecules like arabinose or hydrogen peroxide or ions like calcium and zinc. Some biosensors...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...
Showing: 501 - 520 of 895 results