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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 501 - 520 of 711 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV....a large intervening deletion. Not only can this method precisely program large genomic deletions of up...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks."Molecular...

  3. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...

  7. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas... successful applications, I can't always find something that made them better than my unsuccessful ones...

  8. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...a very positive experience, also let us know! Sometimes those few “thank you”s are what get a customer...

  10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...will update information on the plasmid’s page. Sometimes we’re missing a piece of the plasmid There are...make available as part of our OpenBio Repository sometime soon. Stay posted!     References Guerin K, ...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    .... L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https:...

  12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...

  13. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...transcripts. I remember when small RNA was just something we ran off the bottom of the gel so it wouldn’...thought. High throughput transcriptome sequencing methods including work done by the ENCODE project consortium...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...printer, a laser cutter, woodworking tools, and a metalshop. After two summers in Silicon Valley as a student...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...on any challenge and quickly become experts in something, even if that thing is opening a pizza joint. ...analyze what we want out of our careers: to build something new and beautiful, to engage with the public in...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...from contaminants using a variety of purification methods. Deciding which expression system to use depends...transiently or through stable cell lines. Both methods produce high protein yields if transfection is ...

  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...down the question you were trying to answer, the method you used, the main result, and the fundamental ...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

  19. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...the circulatory or lymph systems to spread, or metastasize. Many cancerous cells will die trying, as the...

  20. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...these electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these... described systems. Several adaptations of this method have been to tag, cage, uncage, and trap the chemical...
Showing: 501 - 520 of 711 results