We narrowed to 716 results for: Met

...bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV....a large intervening deletion. Not only can this method precisely program large genomic deletions of up...

... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

...individuals with similar ideas to actually do something about it. Building a diverse team with different... industry partners, not the other way around. Sometimes, due to misconceptions and stereotypes, solving...

...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...before it was cool or a necessary result of the #MeToo movement. We are carefully attentive to genuine ...

...al, 2017) and cannot be described by a single parameter. Therefore, it is necessary to know how the fluorescence... (iii) attached to a substrate. These in vitro methods allow for a well-controlled environment, but they...

...are no explicit instructions on what to do when something fails. While we’re sure our techs could figure...the contents of a tube with the tube itself. If something happens to the worm that requires a change of ...

...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...

.... L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https:...

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...on-target base editing functionality.  The delivery method of base editors plays a role for off-target editing...

... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...Staudt N, Wright GJ (2010) A rapid and scalable method for selecting recombinant mouse monoclonal antibodies...

...will update information on the plasmid’s page. Sometimes we’re missing a piece of the plasmid There are...make available as part of our OpenBio Repository sometime soon. Stay posted!     References Guerin K, ...

...glycosylation (CDG) are a group of rare inherited metabolic diseases with severe and life-threatening consequences...disorders of glycosylation (CDG): a review. J. Inherit. Metab. Dis. 39, 765–780 (2016). PubMed PMID: 27393411....

... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...

...these findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas... successful applications, I can't always find something that made them better than my unsuccessful ones...

...answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it...a very positive experience, also let us know! Sometimes those few “thank you”s are what get a customer...

...transcripts. I remember when small RNA was just something we ran off the bottom of the gel so it wouldn’...thought. High throughput transcriptome sequencing methods including work done by the ENCODE project consortium...

...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...printer, a laser cutter, woodworking tools, and a metalshop. After two summers in Silicon Valley as a student...

...on any challenge and quickly become experts in something, even if that thing is opening a pizza joint. ...analyze what we want out of our careers: to build something new and beautiful, to engage with the public in...

...degron tags can offer over other protein control methods and compare the commonly available types of degron...protein post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate...