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We narrowed to 850 results for: NTS

Showing: 501 - 520 of 850 results

  1. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These days... makes it much easier to knock-in LexA binding elements into the target region. However, it struck me ...Throughout your career, you have held research appointments in the US (New York University School of Medicine...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information... and memory retrieval. In neuronal tracing experiments, brain tissue is injected with compounds that...injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...

  3. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...significant, negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent...forward for CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The... approach to gene replacement therapy for most patients. But how does one go from “cut and paste” to “...Programmable Addition via Site-specific Targeting Elements (PASTE). Finding the right integrase for this ...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...which people were asked to create a device to help students during an interview. When they were instructed... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...have plans in place to be able to continue our experiments. Thinking about these plans before you’ve run...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...sequencing and bioinformatic technologies are used.  Improvements to sequencing technologies and bioinformatic... lot of effort to stay up-to-date with these improvements. In this post, we discuss the sequencing technologies...addition, DNA synthesis stops. The resulting DNA fragments are pooled and then separated by size using electrophoresis...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

  7. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the...repeating the exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor...). What do Addgene AAV titers mean for your experiments? Because (physical) AAV titers are approximations...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to...Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How We're Sharing ...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...biorepositories or have been directly isolated from patients. In these cases, a test known as Short Tandem ... the lower the passage number the better for experiments. This is to ensure the cells are as close to ...original Cell line you purchased, keeping all experiments carried out on low passage cells and this means...and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist at...

  11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...smaller fragments of the transgene have been seen with the hybrid method. While these fragments are likely...co-infected with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

  12. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...have considered myself neutral in any of their disagreements. In fact, conflict resolution isn’t just about...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making... around missed tasks, stalled projects, and disagreements over prioritization. The source of those conflicts...

  13. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...fun is building within the constraints given. And there were many constraints during the COVID-19 pandemic...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth filming in ...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if...target time frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice... and data professionally). Do remember the fine points of visual aids. Here’s a great slideshare with ...sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone else have...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ...gRNAs. As with other homology-directed repair experiments, you’ll want the guide cut site to be as close... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...

  17. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    .../error, variance, data ranges. how closely data points fall to each other (see Fig. 1). Alpha values...certain information about. The assumptions and requirements that need to be met for a parametric test are... - Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way ... were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the independent...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...are often little tricks that are essential for experiments but that nobody talks about. After months of ...A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9...good gel image. If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands... quick and easy it is to share information and regents, it’s exciting to think that no matter who you ...

  20. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...AAV experiments to the Data Hub. Submit your data by Nov. 12 to compete. The top ten participants will...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...
Showing: 501 - 520 of 850 results