...smaller fragments of the transgene have been seen with the hybrid method. While these fragments are likely...co-infected with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

...have considered myself neutral in any of their disagreements. In fact, conflict resolution isn’t just about...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making... around missed tasks, stalled projects, and disagreements over prioritization. The source of those conflicts...

...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...

...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ...gRNAs. As with other homology-directed repair experiments, you’ll want the guide cut site to be as close... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

.../error, variance, data ranges. how closely data points fall to each other (see Fig. 1). Alpha values...certain information about. The assumptions and requirements that need to be met for a parametric test are... - Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way ... were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the independent...

...are often little tricks that are essential for experiments but that nobody talks about. After months of ...A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9...good gel image. If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands... quick and easy it is to share information and regents, it’s exciting to think that no matter who you ...

...parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...

...AAV experiments to the Data Hub. Submit your data by Nov. 12 to compete. The top ten participants will...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

...CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. Special thanks to our...enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly...

...capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent widefield microscope...your institution when heading into a new set of experiments. Many thanks to our guest blogger, Doug Richardson...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent...

...scientific communities and welcome suggestions in the comments.  X has moved significantly away from the functionality... Every community benefits from a diversity of viewpoints, and we acknowledge there may be a variety of...invite you to follow us across our social media accounts.  -Addgene   ...

... Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them thoroughly...before use.  For efficient mixing, add the various reagents to a single tube and simply flick the tube several...everything Use barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always...

...removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil ...developed two fusions of a CBE with other BER components: the DNA repair protein rXRCC1, or the DNA binding...both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE and cOPTI-CGBE had...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...

...Tom McFadden, recently worked with high school students in the bay area to create a plasmid rap video ...has made many more Science rap videos to teach students around the globe and is pushing SciComm further...DNA. 2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African...

... Below, we break down each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In...learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast Segment, or have...for new Hot Plasmids topics, let us know in the comments section below. Hot plasmids covered in this segment...

...AAV packaging experiments. Let us know what you think about the calculator in the comments section below... do the math for you and it’s simple to make adjustments for the type of tissue culture dish and number...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...teaching. He hopes to teach his molecular biology students about the functions of these powerful tools and...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

... to carefully-screened research and teaching departments at universities around the world. Connecting ... assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s...incredibly important for scientific breakthroughs, reagents, and know-how to transcend national borders and...

...URL, same great articles to help you with your experiments, but a new look. We started the blog in 2013... community, share scientific discoveries and developments, and provide tips and tricks for new and seasoned...emailing us at [email protected] or tell us in the comments below. We’ll track down the story, get the inside...

...about their research, their job hunts, and their tips for experiments. We’ve also loved helping to answer... Plasmids 101 series and by responding to your comments. Additionally we’ve seen what topics are of interest...