We narrowed to 517 results for: PROC
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TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and...
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Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....Red-mediated recombinants in mismatch repair mutants. Proceedings of the National Academy of Sciences of the United... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....to study gene regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....purposes, antibodies can be used to affect cellular processes in vivo, and these are referred to as functional... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... Encouraged by the high efficiency and easy procurement of cr:tracrRNA reagents, we scaled up these tagging... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....alternatives to Cas9. Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....genes, Green Listed can help make the gRNA design process quick and easy. Green Listed - a CRISPR Screen ... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United... -
Which Fluorescence Microscopy Techniques is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....with deconvolution, a mathematical software post-processing step that reassigns out of focus light to its... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences 96.5 (1999... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....timing, and reversibility of the genome editing process. Read on to learn about the various light-controlled... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m.... light to alter cell physiology and molecular processes via genetically encoded proteins. PhoCl is a new... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m..... They can be stored at your bench during the procedure and at 4 °C when you’re not running a western.... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....high background or low signal during the imaging process, try adjusting your exposure time first, since ... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction levels...