We narrowed to 599 results for: REV;
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TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....suggestions. Most coaches have websites you can review and most create useful online content in the form...
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Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....regulatory molecules, and has provided clues to their previously uncharacterized cellular roles. In the current... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....choice affect my experiment? In this post we will review some of the main factors that affect isotype choice... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....proteins available. Fortunately, several excellent reviews have collated data from various papers to make ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....issues.” We then ask the depositing laboratory to review the discrepancies. If the depositing laboratory... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....adult-born granule cells over time that were not previously possible. Bensussen S, et al., iScience 2020... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...