We narrowed to 580 results for: SIM
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TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies...
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xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....examine your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m.... design to analyzing results. To use this tool, simply follow these steps: Create a new experiment in... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m.... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m.... and requesting each year (Figure 2). We did a similar analysis with CRISPR pooled libraries, although... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....which target E3 ligases to specific proteins by simultaneously binding both their target protein and an E3... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....for the foreseeable future. These newer findings simply add to our understanding of how this antibody works... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can be purchased 5’-modified from... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....depositing lab. There may be other plasmids with similar names in the collection, but you can use this name... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....transparent organisms. Advances in tissue clearing Similar to light microscopy, there have been significant... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....organisms with Cas9 risk creating a gene drive? The simplest way to make a gene drive is to insert an endonuclease... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...