We narrowed to 813 results for: TIM

...find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors CRISPR toolkit...rhamnose-inducible promoter. While this system was optimized for Burkholderia, they identified 67 genera of...

... and more open to developing a relationship. The time to develop relationships is before you need them...that is helpful. Keep in mind that this is a good time to ask personal questions about salary, opportunities...

...play a role in the progression of cancer are often time consuming and expensive, especially when done in...functionally validated. In vitro assay allows first-time functional annotation of thousands of mutations ...

...infect only one or a few species of bacteria and sometimes, only a subset of strains of the same bacterial...researchers in the directory are volunteering their time and resources and there is a chance that an early...

...published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP...already been made, and it would be expensive and time-consuming to repeat the process with these new photoconvertible...

...what they need - whether it's their first or 500th time on addgene.org. Additionally we also wanted to highlight...look and explore the new homepage. We hope every time you come to Addgene to find the plasmid or viral...

...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the...

...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher...

...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions...

...  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools, beautiful...beautiful questions, and smart scientists.  At the same time, he pointed out, academic research has some big ...

... we can’t localize one cell at a depth of one centimeter with fluorescence is because the light becomes...relevant papers, and then read them again. Most of the time, the answers will be there.     References: Bright...

... purification is still possible but requires optimization and often more stringent washing. Nonetheless...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

...cancerous, hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human...who got it from who knows where), now is as good a time as ever to find out the true identity of the line...

...fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. ...bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic gene 1 (arg1...

...making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids... to quickly align multiple sequences at the same time. This means we can easily compare and analyze any...

...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...

...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

...mice to gather the following types of data: Report time-course and concentration of released DA in mouse... dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish Report DA release...

...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates...

... still what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.”...department, and operations department with the ultimate goal of determining if projects are feasible. ...