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We narrowed to 813 results for: TIM

Showing: 501 - 520 of 813 results

  1. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...find more new CRISPR tools for your research! This time: CRISPRi in Burkholderia Base editors CRISPR toolkit...rhamnose-inducible promoter. While this system was optimized for Burkholderia, they identified 67 genera of...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...Nature. Our take home: users likely need more time to optimize and troubleshoot the system. Site Directed...

  3. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... and more open to developing a relationship. The time to develop relationships is before you need them...that is helpful. Keep in mind that this is a good time to ask personal questions about salary, opportunities...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP...already been made, and it would be expensive and time-consuming to repeat the process with these new photoconvertible...

  7. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...what they need - whether it's their first or 500th time on addgene.org. Additionally we also wanted to highlight...look and explore the new homepage. We hope every time you come to Addgene to find the plasmid or viral...

  9. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher...

  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions...

  11. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools, beautiful...beautiful questions, and smart scientists.  At the same time, he pointed out, academic research has some big ...

  12. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ... we can’t localize one cell at a depth of one centimeter with fluorescence is because the light becomes...relevant papers, and then read them again. Most of the time, the answers will be there.     References: Bright...

  13. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... purification is still possible but requires optimization and often more stringent washing. Nonetheless...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...cancerous, hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human...who got it from who knows where), now is as good a time as ever to find out the true identity of the line...

  16. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibodies in llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller...name.  We’ve talked about sdAbs a number of times on this blog over the years and have described how...

  18. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. ...bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic gene 1 (arg1...

  20. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...
Showing: 501 - 520 of 813 results