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We narrowed to 816 results for: ache

Showing: 501 - 520 of 816 results

  1. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We...for at least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do...

  2. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...points to collaboration and delegating as keys achieving success. Chapter 9: Science by the Pint - We learn...knowledge and continuity - two lead organizers on each program, one junior and one senior.  To learn more...

  3. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...Albert G. Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on...

  4. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned...The Addgene lab often works as a very well-oiled machine and this post was no exception - the rest of the...

  5. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well and communicates clearly...without ever being demeaning Does not micromanage (teaches you to fish, doesn’t fish for you) Helps identify...

  6. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...expected titers, a customer support specialist will reach out to you during the request stage, before you ...  Rachel Leeson...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...they would be disregarded - everyone was very approachable.     Fig. 1: Examples of DNA sequencing...was done through the “Addgene 101” series, where each team gives a presentation on what they do and how...

  8. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...property rights and handling your constructs once they reach you. As with all of our resources, we will continually... our website; towards the bottom right corner of each page there’s a blue icon displaying the word “Help...

  9. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...variety.” In most cases, backbones are available to attach the gene of interest to either the N-or the C-terminus...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...inquiries. The office is now focused on scientific outreach and community engagement. Information about contacting...Just a few weeks ago I became the newest Addgene Outreach Scientist - the first to be located in London ...

  11. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

  12. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

  13. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

  14. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...plasmids in a number of different experimental approaches. They first assessed the activation of different...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

  15. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  16. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

  18. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...step cloning, and overlap extension PCR. Although each type of cloning has its advantages, most scientists..., Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She is also a...
Showing: 501 - 520 of 816 results