We narrowed to 884 results for: ica;

...progress on establishing a system for the chemical replication of RNA in the absence of evolved enzymatic...enzymatic machinery (i.e. RNA replication with no proteins). The chemistry is complex and this work requires...inheritance that might be less error prone than DNA replication. He too mentioned how “surprising” small RNAs... about using CRISPR/cas to address the growing clinical problem of antibiotic resistant bacteria. See ... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...Steitz quipped that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring...

...Because of their small size, nanobodies can be synthetically expressed off of plasmids in bacteria, and they...with a large and diverse pool of sybodies that hypothetically contain ones that bind strongly to your target...Starting with libraries and a biotinylated (for purification purposes) protein target of interest, the protocol... identifying sybodies that bind strongly and specifically to the protein of interest. The Seeger lab used...-30) of sybody candidates are sequenced for identification. All unique sybodies can be subcloned into ...2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741. https...Light to Manipulate Cell Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids...

...McDonald. "A practical guide to evaluating colocalization in biological microscopy." American Journal of... proteins of interest and examine them using biochemical methods thus removing the context of the cell...way it is important to choose the appropriate quantification methods and tools. It is usually not enough...microscopy is limited by the wavelength of light and, practically speaking, for common lab microscopes the detection...Quantitative analysis of dynamic association in live biological fluorescent samples." PloS one 9.4 (2014): e94245...

... (green or blue) within a population of timer indicates the age of the pool: the higher the ratio, the...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...protein, or cellular structure and a particular biological event (trafficking to a subcellular location,...of neural fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed ...to red report on cellular trafficking." Nature chemical biology 5.2 (2009): 118-126. PubMed PMID: 19136976... insulin-secreting beta-like cells." Nature communications 7 (2016). PubMed PMID: 27063289. PubMed Central...

...requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few...survey. When asked if they could detect indels (an indication of gene disruption ability) with NgAgo, one respondent...could not achieve indels with the NgAgo system indicates that it is very tricky to use and fussy to optimize...proposed in the original article, to achieve greater efficacy, researchers will have to carry out repeated transfections...results out there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

... most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin...capacity. Many of these FPs are fluorescent when ectopically expressed in most organisms. Furthermore, fusing...fluorescence. Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to...For LSS proteins, the Stokes shift is ≥ 100nm. Specifically, these proteins are excited by UV light or blue...were prone to oligomerize. This may affect the biological function of the FP-fusion protein. Therefore,.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

...excited to announce we now offer an export license application for rabies materials! Some of the materials in...international requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control...will need to provide a brief description of the applicable research project when purchasing export-controlled... applied to cover processing costs. Once the application is approved, Addgene then ships the material ...offered VSV and SARS-COV-1 plasmids under this application process since May 2020. We find this process ...whenever possible, as we cannot expedite the application process.  More information about export control...

... antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ... antimicrobial agents in MIC trays. Journal of Clinical Microbiology, 23(5), 959–961. https://doi.org/...

...translational applications (mechanistic studies and gene therapy treatments for neurological diseases). ...neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...astrocytes. Why screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but...throughout the body and is often difficult to surgically access. Therefore an IV injection is an ideal...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...

...that, they also help us keep track of the tubes’ physical location in the lab. These barcodes additionally...  freezers depending on the sample type. It is critical for us to know which plate, rack, and freezer ...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...help us track each sample, but we also have a dedicated and detail-oriented lab team that works carefully...performs an average of 140 transformations. The lab typically transforms newly deposited samples from Monday...Excel sheet.3. Be specific  - When assigning a physical location to your samples, include as much detail...

...Summer Science Communication Series. We’ll be covering the basics of science communication, tips and tricks...science with others, and introducing science communication as a research field. And there will even be ...be a few tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...parenting strategies and make important ecological indicators since species live in water and on land....protocol community! Asclepias syriaca and Asclepias curassavica (Gerog Jander) Milkweed plants are known to many... as the only Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds...Lab (@EvoEmbryo) September 24, 2018   Xenopus tropicalis (Gary Gorbsky) This is not a new experimental...plants and are unusual enough to be interesting biologically. They have economic importance as harmful weeds... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...folding and stability, change post-translational modification sites and even affect protein function (Mauro...recognize multiple synonymous codons) may actually be critical for determining the rate of translation. The ribosome...10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization in human therapeutics...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org... GluCl ion channel genes for mammalian cells dramatically improves expression levels. Journal of Neuroscience...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...

...described above showed successful target modification, indicating that AAV is a good delivery vehicle for...overall packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so without a substantial immune...enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain of RNA ...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...

...efficiency. This can be done through domestication, which is the modification of a DNA sequence to remove a ...This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi... the synonymous mutation of a codon. You can domesticate the sites yourself using PCR, but gene synthesis...gene synthesis, you can request the sites to be domesticated through the process, and request your sequence...high-throughput, we recommend transforming into chemically competent cells in a 96-well PCR plate. Always...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ...

... this post I’d like to delve deeper and look specifically at the characteristics and capabilities an ELN...facilitate true reproducibility. The following are critical: (1) connectivity to other data sources used in...likely stored in institutional and lab servers specifically designed to hold large amounts of data. Although...group’s research – it makes no sense and is not practical to move all this data in bulk into a lightweight...lightweight tool like an ELN. It is nevertheless critically important to be able to conveniently reference specific...with a particular experiment or project thus significantly enhances the ability of others to subsequently... both accept deposit of data associated with publications into one of five general purpose open source...

... gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...asymmetric target site core but its 8 bp length that is critical for Cre and FLP function. Because recombination...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...this plasmid mix results in sparse labeling of cortical neurons and their synapses.   How can you...control   Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry...(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces...

...very little data on which might work in your application. It can be quite difficult to decide on which...be searched and sorted by gene/target, name, application, pass/fail rating, sample species, antibody species... how the antibody performed in the submitted application, along with a brief description of the experiment...experiment. If that data was included in a publication, you'll find that information in the report as well... section will vary slightly depending on the application — flow cytometry, western blot, immunohistochemistry...data showing how the antibody worked in your application. The submission process is easy, with simple,...

...window onto communication between the muscle and brain, which may have therapeutic implications, say Hyo ...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands,” ...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their..., the brain is protected from stress-induced biochemical changes that could otherwise lead to depression...

...speak for you (and you for them) Communication Being able to communicate effectively is part of what makes...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop...productive. Take all the opportunities you can to be communicating about science with others. If opportunities ... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...