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Showing: 501 - 520 of 669 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest...transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...positions without changing the order of the amino acids in the protein) may be able to boost FRET efficiency...

  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...affect nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop...CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198...

  5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...

  8. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic Molecular Biology. Elsevier, pp ...for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...

  12. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...

  15. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...Isolation of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in Tissue ...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 ...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins...

  19. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ....org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ...
Showing: 501 - 520 of 669 results