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We narrowed to 759 results for: nes

Showing: 501 - 520 of 759 results
  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...
  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...
  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...
  4. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...
  5. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...
  6. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...
  7. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...
  8. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...
  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...
  10. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...
  11. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second...between technical and managerial roles, and I've witnessed several colleagues do this while changing industries...
  12. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...the different populations. MFI is the average brightness from all cells that are positive for the marker...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...
  13. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...
  14. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...
  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...
  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...
  17. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...
Showing: 501 - 520 of 759 results