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We narrowed to 466 results for: SHI

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Showing: 261 - 270 of 466 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...

  2. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... 8760896. PubMed Central PMCID: PMC146030. 26. Nishida, Hirokazu, et al. "Overexpression, purification...Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR...

  3. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands...critical features based on my own experience: Leadership Few things are more frustrating than being on...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements...

  9. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...
Showing: 261 - 270 of 466 results