...about their research, their job hunts, and their tips for experiments. We’ve also loved helping to answer... Plasmids 101 series and by responding to your comments. Additionally we’ve seen what topics are of interest...

...fueled by highly caffeinated grad students and postdocs, which accounts for many, many lattes and espressos...infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have...

...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...is used by the community to drive new science experiments. Finally, we were thrilled by how many people...from the expansion of our Data Hub to process improvements in every aspect of our organization. One thing...

...tools from depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV...began working with tech transfer offices to make reagents available to scientists in both academia and industry...Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset...

... be used for A•T to G•C edits in protoplasts or plants of rice and wheat. Read the paper in Genome Biology...cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in diverse bacteria Carol Gross’s lab recently...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...us to make organs using iPS cells derived from patients. Chapter 8: The importance of a mix of research... disease. How Darrell is using stem cells from patients to study various lung diseases. Chapter 10: How...and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity...

... strong as the basis for many genome editing experiments. So, without further ado, the most request plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

...Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...to get, as well as give, information to make improvements. Members connect directly with one another...in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many ...

...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...would be a wonderful tradition for university departments to establish, with the goal of fostering better...

...This represents a considerable barrier to furthering research and developing effective treatments for ...dozens of wild-type genes and their associated mutants in other model systems. It is possible that a given...

...scientists quickly find much-needed phages for patients.   A phage directory is crucial because it's hard...Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, it's likely that...in the works. To keep up to date with the new developments at Phage Directory and in the phage field, subscribe...

...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

...At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade...which has already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has...you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually...

...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...specific filter sets and experimental output requirements. Select from these yeast-optimized fluorophore...vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others may be interested...

...action - Search! Along with this we’ve also made improvements to our global navigation and menus over the ...built on the foundation of a community that shares reagents and knowledge with one another, and Addgene make...content above the fold, we decluttered the page elements and allowed our content to dictate the space. ...

...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...time. This made time for me to attend to other commitments, without feeling guilty about missing work. As...Northwestern University. She is one of several students completing their co-op at Addgene. This is her...

...attractive imaging platform for in vivo imaging experiments because  ultrasound devices are inexpensive, ...coli strain Nissle 197 (ECN) was used for these experiments because it’s a probiotic strain that can colonize... and inflammatory bowel conditions. In these experiments, arg1 expressing ECN were injected into the mouse...

...studies rely on delivering reversible or varied amounts of stimulation to receptors. Optogenetics offers...deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR If precise spatiotemporal control of...brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan Roth lab for feedback on...

...of your sample over time and determine which experiments you can effectively use the DNA. pH and DNases...protein degradation, and/or the use of chelating agents that can be introduced in the purification protocol...without having substantial degradation and which experiments you can use your plasmid.  DNA elution choices...

... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...