We narrowed to 813 results for: TIM

...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

...antibodies in llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller...name.  We’ve talked about sdAbs a number of times on this blog over the years and have described how...

...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...

...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates...

...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...this format and it becomes even more effective over time if you take it seriously.  Person who will receive...

...genome editing toolkit. However, a challenge in optimizing base editing technologies across several cell... APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate...

...individuals interested in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] Arabidopsis to your class for the first time – we want to hear from you. Reach out to us at ABRCedu...

... without the need for cellular HDR.   Having had time to digest my notes, since the conference, I think...cut-to-mutation distance affects editing efficiency. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ...

... experiment has not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

...neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your...

... replicate, and the transgene will be lost. Over time, bacteria containing the recombined vector backbone...post. Check yourself before you recA yourself. Optimize Growth Conditions To reduce recombination, it ...

...you have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

...tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ...

... or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used ...

...community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew...

...Addgene, with more added to the collection all the time! We’ll serve up a sampling of some of the most popular... what these libraries are capable of at the same time. Barcoding libraries Just as physical barcodes help...

...gene synthesis, many researchers now use codon-optimized versions of common coding sequence features, so... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...

... be spending less time and resources resupplying our current inventory and more time providing new materials...

... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI...

... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially...