We narrowed to 803 results for: TIM
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TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m.... be spending less time and resources resupplying our current inventory and more time providing new materials...
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Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....genome editing toolkit. However, a challenge in optimizing base editing technologies across several cell... APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....individuals interested in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] Arabidopsis to your class for the first time – we want to hear from you. Reach out to us at ABRCedu... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m.... without the need for cellular HDR. Having had time to digest my notes, since the conference, I think...cut-to-mutation distance affects editing efficiency. Optimizing Donor DNA for Enhanced CRISPR Genome Editing ... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....gene synthesis, many researchers now use codon-optimized versions of common coding sequence features, so... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....you have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m.... experiment has not worked…again…for the seventh time…. I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m.... replicate, and the transgene will be lost. Over time, bacteria containing the recombined vector backbone...post. Check yourself before you recA yourself. Optimize Growth Conditions To reduce recombination, it ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m.... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m.... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... working on several genetic diseases at the same time. The limitation of budget restrained us from costly... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...to try these optogenetic tools out for the first time? Boyden: There are always considerations for each... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m.... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....understanding of PZA action and could potentially stimulate the development of useful diagnostic methods and...non-linear microscopy methods will be used to stimulate and manipulate neural populations in order to ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying...are verified and identifiable to save researchers time and make their work more reproducible. While Addgene... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ...