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Showing: 521 - 540 of 669 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent...downloads/modules/FRET_teaching_module.pdf Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: ...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... recombinant adeno‐associated viruses." Nucleic acids research 32.1 (2004): e3-e3. PubMed PMID: 14704360...endogenous genes in diverse human cell lines." Nucleic acids research 36.19 (2008): e127-e127. PubMed PMID: 18784188...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice from ... copied below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect...

  7. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them....a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the office....

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...professionally). Do remember the fine points of visual aids. Here’s a great slideshare with more details on ...question. I like to ask about how many people use plasmids in their research and then see how many of them...

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...they expressed well, the proteins encoded by the plasmids in the library will remain attached to the membrane...of Expression Vectors Deposit Your Microbiology Plasmids ...

  12. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...

  13. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...Visit the Fluorescent Protein Guide Find antibody plasmids for your experiments ...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...efficiency from a single reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A...Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages...

  16. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...deposit with us!  This year alone, we had 12,427 plasmids deposited with us. We cannot say thank you enough...

  17. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ... helps scientists understand how to use CRISPR plasmids in the lab and the vast range of possibilities...

  18. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000...
Showing: 521 - 540 of 669 results