We narrowed to 845 results for: CAL;
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TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...
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Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....expression pattern of a defined promoter region, ectopically expressing YGOI in a pattern that allows the ... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....benefits to researchers conducting basic or early pre-clinical research. However, standard terms and techniques... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....Although the sequence and regulation of the ori dramatically affect the copy number of a plasmid, other external... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....AAV viral preps to easily find and share useful technical data. These data focus on experimental design ... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than the RNA guides employed by... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....Roth’s lab are already available allowing you to chemically control neuronal activity while studying your... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m.... that will bring about the next revolution in biological research. We’re incredibly happy to see that ... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m.... or pink. Purpose Gram staining can be used in medical settings to determine the general type of bacteria... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....incredibly useful, yet simple system allows you to systematically study gene function with temporal control and... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....ensures that everyone gets a chance to talk and less vocal team members still have their voices heard. Here's... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the best... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m.... and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....in target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what do you do? — Stephani Page, ... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m.... disciplinary communities gather at ComSciCon's local and specialized franchise workshops throughout the... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....m always catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....need to determine these transduction patterns empirically for their own experiments. To this end, we offer... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing! A recent... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....information is organized in wiki format and includes biochemical properties, previously established uses, and ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m.... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...