We narrowed to 659 results for: Clu

1. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...DREADD (Kügler et al., 2003), which can then be exclusively activated by the small molecule clozapine N-oxide...

2. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ...neurons. We have expanded our AAV collection to include two new ChR2 constructs that allow for targeted...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural proteins 1 to 16, spike...

4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG...

5. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...team, we identified what applications should be included and what basic information someone new to antibodies...

6. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...Things to consider when selecting an assay system include: How specific is it? Is it high throughput? Will...

7. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ... remains a challenge due to multiple factors, including limitations of number of flourescent proteins ...

8. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...into DNA expression and protein interactions, including epigenetic modifications. But it's not an approach...

9. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...commercial antibodies come with sodium azide already included in the buffer, which will be noted on the product...

10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type

    Blog Post

    Published

    Aug. 19, 2025, 1:15 p.m.

    ... a deeper dive into the science behind OCaMP, including detailed characterization as protein or in neuronal...

11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...the slower exonuclease activity to proceed. By including only one type of dNTP in the reaction mix, the...

12. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ...The information is organized in wiki format and includes biochemical properties, previously established...

13. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ... transcription of other relevant viral genes (including the ones listed previously). E3/E1B suppresses...

14. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ... the antibodies you are using, you may need to include additional controls like FMO or isotype controls...

15. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...been approved in the US for multiple diseases, including hemophilia B and spinal muscular atrophy.   ...

16. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...panel shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of ...

17. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ...and cover letter revised by your network, which includes your friends, family members, and professional...

18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...described in this post in the graphic below - including some simple comparisons. The easy multiplexing...

19. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...NHEJ has a vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis...

20. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...Further Reading: For more information about SAM, including a tool for getting optimized guide sequences for...