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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 798 results for: Cre

Showing: 521 - 540 of 798 results

  1. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-fold. Read the paper ...variants with mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold...

  2. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used by the community...understand our role in opening up science to help create new knowledge and research opportunities. I have...

  3. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely...inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28 and the chaperone...

  6. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...into our lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways...learn that it hasn’t even been 50 years since the creation of the United States Environmental Protection ...

  7. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... describes an ingenious but simple technique to screen for proteins that could be useful for a wide variety...event gives me the opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our...

  8. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...can almost be boiled down to one, key concept: Creating a culture of excellent, effective communication...practice and training. This is important because it creates a culture of engagement in the issues at hand and...

  10. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... are we! This expansion will give researchers increased flexibility when planning their research and expanded...This split allows us to provide researchers with increased choice and flexibility; while ensuring we can ...

  12. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... to GRABNE, and having both colors allows for increased options for multiplexing in experiments, including...both nLightG and nLightR showing significantly increased selectivity for norepinephrine over dopamine. ...

  13. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

  18. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

  19. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

  20. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary...
Showing: 521 - 540 of 798 results