We narrowed to 863 results for: ELL

...families of cell surface and secreted proteins (glypicans, integrins, and more coming soon) as well as epitope...high-throughput screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative...for comparing relative affinities across mutants. Cell surface display systems, such as bacterial, yeast...presentation of your mutant libraries on the surface of cells. This enables direct screening via flow cytometry...therapies and improve their delivery methods to various cells. The wealth of smaller engineered antibody fragments...

...the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check out...AAV9-X1.1 enables expression in brain endothelial cells via an injection into the systemic circulation. ...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also...recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via newly-...before. We have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA,...

...two-photon microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   ...voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in deep tissue, in...voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here (and here...human c-myc proto-oncogene product. Molecular and Cellular Biology, 5(12), 3610–3616. doi: https://doi.org...

...circuits are generally linked to dopamine (DA), a well-known reward chemical and neurotransmitter. But ...firing patterns (or the frequency of its release) are well understood, the direct effect of DA binding to a...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

... remember what happened when drug X was added to cell line Y, or the phenotype of a mouse with a specific...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make...the right experiment! The ReFigure extension also tells readers if a paper they’re reading has been linked... to show that a previously used reagent performs well, or you might publish a replication experiment that...immuno-oncology. Funding agencies such as the NIH and Wellcome Trust now accept interim research products (pre-registration...

...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ... Addgenies like the nonprofit company culture as well. We work every day to do a better job of enabling...Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, Lianna, Luke, Marcy, Mary, Max...Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal...Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease...

...sweat, and tears, as well as the whole point of the paper. They should be able to tell your story for you...I am writing isn’t well written, I must write everyday in order to get better. Well what does that mean...interaction of critiquing others’ manuscripts as well as being critiqued added an extra level of application...

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...about recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access ...resource of plasmids encoding recombinant antibodies as well as other affinity reagents such as single-chain ...need for an open-access, molecularly defined, and well validated collection. I’m thrilled to be working...

...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage... a common thread at the meeting. The new Somatic Cell Genome Editing program, run through the NIH Common..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...Improve HDR Efficiency for CRISPR Editing in Human Cells Hands-on tips for repair template design from guest...

...R-factors could be transferred between bacteria via cell-to-cell contact; however, scientists noted that, unlike...cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity...PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological...Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal of Hygiene 60.3 (1962...

...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...emission spectra of the protein, so that the protein is well excited by your microscope and the fluorescence ...laboratory of Ronald Vale, after which he was a fellow at the Bauer Center for Genomics Research at Harvard...

... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...management skills. Finally, developing emotional intelligence is vital. Learning to "read the room," understanding...part of your broader career development.   Matteo Tardelli authored the book "Beyond Academia: Stories and...molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps and...

...can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...there’s some great reminders for Twitter pros as well, like tip #4: “Follow people for about a week and...

...recognized and valued for its immense importance, it may well be what determines whether you get the job after...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...forget that you have a much bigger job than merely telling your audience about the work you've done. If you...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...in science communication The strength of story telling Science communication: 9 strategies to get your...

...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses...Steroid-regulated LexA promoter XVE (synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature... Because transcription machinery differs between cell types or organisms, promoters are similarly variable...variable. Bacterial promoters only work in prokaryotic cells and typically only in the same or closely related... were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique...

...have lunch or coffee with someone I don’t know very well at least twice a week. I have done this with people... career advice. Your family and friends know you well and might have good ideas for who you should connect...Healthcare Businesswomen’s Association hosts an excellent group mentoring program in many of its chapters...keep working with you is report back with progress. Tell them how their advice made a difference, helped ...in a leap in your skills. If a first meeting goes well, you can say, “This was really helpful, perhaps ...

...system, antibodies are produced by B cells. Each individual B cell produces antibodies that all recognize...also all the same isotype. But together, all the B cell clones in the immune system make different isotypes...antibodies starts with selecting just one of these B cell clones for further antibody production, polyclonal... blood from the immunized animal. The red blood cells and the serum are separated, and the serum - which...

...thinking. Being able to learn things quickly. Story telling. The ability to know what’s a story and how to ...to hook your readers with it.” She feels that storytelling is an impactful tool for science communicators...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece... starting a blog, but with one caveat: “I hate telling people to work for free because you shouldn’t work...social media as a scientist here Learn more about storytelling in science ...

...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...of this to get the information you need to choose well. What are their qualifications? Do they have certifications...does not endorse any of these coaches and I can't tell you which one will be a match for your needs, but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...Industry transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, ...