We narrowed to 776 results for: IRE

1. Using Addgene's New Viral Service for Your Research

   Type

   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ...yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

2. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   Published

   Nov. 12, 2019, 2 p.m.

   ... public and retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

3. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   Blog Post

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   June 17, 2021, 1:17 p.m.

   ...turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about our paper...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

4. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   Blog Post

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   May 25, 2021, 2:29 p.m.

   ...antibody tool and therefore will grow to serve the entire scientific community.     Why recombinant antibodies.... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ...

5. Antibodies 101: Epitope Tags

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   Blog Post

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   May 11, 2023, 1:15 p.m.

   ...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

6. Plasmid Cloning by PCR

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   Blog Post

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   March 29, 2016, 2:30 p.m.

   ...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...

7. Reagent Repositories Are Speeding up Science During the Pandemic

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   Blog Post

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   Aug. 4, 2020, 1:15 p.m.

   ...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research...Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection ...

8. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   Blog Post

   Published

   July 21, 2020, 1:15 p.m.

   ...ml This method is quick and simple and doesn’t require any special reagents. However, there’s limited ...absorbance-based methods, fluorescence-based methods require a standard curve, a set of samples with a known...

9. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

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   June 30, 2020, 1:15 p.m.

   ...audience engaged. They first sent a pre-workshop questionnaire to the participants to learn about what they...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...

10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

11. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

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    July 21, 2017, 12:48 p.m.

    ...tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter sounds...34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression systems...

12. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

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    Sept. 3, 2019, 1:15 p.m.

    ...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...such as transcription factors tethered to dCas9, require long time scales with constitutively expressed ...

13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ...serotonin. Using a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput...anxiety, but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger...

14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

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    June 23, 2022, 1:15 p.m.

    ...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

15. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    Blog Post

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    June 1, 2021, 1:15 p.m.

    ... research projects to a halt. None of us works directly with viruses or transmissible diseases, so we ...and availability; 3) Make the contact to start a direct collaboration between researcher and expert; 4)...

16. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

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    March 30, 2023, 1:15 p.m.

    ... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

17. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...

18. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

19. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...