We narrowed to 793 results for: KIN
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TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....properties (for example, overall brightness or on/off kinetics) that makes them suitable for different applications...with a CAG promoter exhibits high specificity for Purkinje neurons. 105321 AAV1 pGP-AAV-syn-jGCaMP7c...
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Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m.... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m.... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you? If you are looking for a human cell line model, HEKs might be a great... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story. At this point you may be thinking “I don’t want to bury my results all the way ... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....catalog, so that you will know exactly what you are working with. We will also be collecting feedback from ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV: the... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m.... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Incredibly imaginative, funny, bittersweet, and shocking at times." - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic...mimic the human lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...