We narrowed to 610 results for: SCR
-
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....conformationally restricted RNA which improves mismatch discrimination and is more nuclease-resistant, resulting in...
-
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....-independent assays such as mRNA data from transcriptomics; independent antibody strategies that compare... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....secondary will bind to all the primary antibodies indiscriminately, giving them the same output signal. In these... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m.... this DNA structure can readily fold into transcriptionally active double-stranded form through intra-... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....your slide deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....plasmids, using both mass production genome wide screens and the more traditional “one at a time” approach... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....systems, this system incorporates the Tet-off transcriptional activation system to drive high levels of expression... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from rare diseases... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....corresponding S. cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....now. These 9 strategies will get you started: Subscribe to Career Blog Posts from Addgene 1) Twitter –... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....poster format differs in many ways from writing a manuscript. If you have ever attended a scientific conference... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m.... can also deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are ... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...