We narrowed to 612 results for: Sis
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TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....you can often remove the incompatible agents by dialysis or gel filtration. There is, of course, a lot...
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Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....the level of gene expression. For example, the synthesis of deoxychromoviridans requires three genes whose... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification ... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....salary information as of December 1st. Salary inconsistencies across geographical regions When we originally... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m...., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....plant biology to achieve traits such as disease resistance. MoClo is a great tool to deliver many combinations... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of generating... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...