We narrowed to 608 results for: Sis;
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TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...
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RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....salary information as of December 1st. Salary inconsistencies across geographical regions When we originally... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....you can often remove the incompatible agents by dialysis or gel filtration. There is, of course, a lot... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m...., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....backbones expressing blasticidin or puromycin resistance genes. It’s that simple! Good luck and happy... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....positive selection marker such as an antibiotic resistance gene is also included to select for successfully... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m.... codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes...