We narrowed to 543 results for: TIL
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TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....will keep cutting and repairing your target DNA until a mutation is introduced disrupting one or both ...
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Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....Additional Resources on the Addgene Blog AAV: A Versatile Tool for Gene Expression in Mammals AAV Titers... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....preparing your talk early - like today Don’t wait until you need to speak to start creating slides or thinking... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....University. Site directed mutagenesis is a highly versatile technique that can be used to introduce specific... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....negotiations can be much easier. However, negotiations can still go horribly awry when several factors aren’t taken... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes. Scientific Reports, 15(... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m.... a mouse IgG2a isotype primary antibody but you still observe a signal from your anti-Mouse IgG2a secondary... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile Expression System for Drosophila. Methods Mol ... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....hear too many science trainees get warnings and still think “but that won’t happen to me, I have a backbone... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....designs, we have observed that certain GRSMs may still not perform as expected, perhaps due to idiosyncrasies... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....common problems that many others have faced yet, still, this challenge did not hinder them from advancing... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development: the power of optogenetics... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m.... A new positive selection-based RNAi method Until now, all DNA-based RNAi experiments typically involved... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....secret about science policy that I didn’t learn until arriving here in DC, is that so much policy actually... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....Resources Additional resources on the Addgene blog: Still trying to choose an antibody? Check out our Selecting... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....have - sometimes you just don’t know who you need until the team really starts functioning (or malfunctioning... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded breaks (Tsai ...