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...introduce any one of these topics without some editorializing, and he wisely makes no such attempt. He is...discusses have been making the rounds in journal editorials, science social media, and conference workshops...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

...CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator design that consists of inactive... domains that recruit additional transcription factors. The Root and Doench labs utilized tracrRNAs with...stem loops to recruit additional transcription factors to the targeted region (Sanson et al., 2018) . ...tracrRNA was combined with the sgRNA expression vector allowing the screen to be conducted with only two...

... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates... coveted Blue Flame is more than an award for depositors and inspiration for our updated mascot, it’s ...model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you planning?...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

...2020). In the two-plasmid system, an effector plasmid (pEffector) expresses all seven required genes and...CRISPR-Cas systems, in contrast to single-protein effectors employed by Type II systems (such as CRISPR-Cas9...plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

...acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength in the... forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires...that the protein could be taken directly from A. Victoria and expressed in any organism while still maintaining...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...always get done, but you’ll have time to read a story to your baby or run that extra two miles which is...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...requiring either expressing it with additional cofactors or using significantly truncated forms that limit...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

...much information as possible from depositing laboratories and associated publications, but our quality...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...user interface, so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for ...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS ...

...motifs within transmembrane proteins, such as receptors, or post-translational modifications on those ...) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells and...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note that when.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...equipment, Fluorescent emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

...genetically encoded fluorescent probe. Several factors contribute to the popularity of GFP including (...organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter ..., proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains that...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein binds...Smaller genetically encoded probes are based on cofactor binding proteins as described above. The smallest...

...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record...means to manage their work, combining data, team, inventory and project management functionalities with experiment... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

... AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot ...AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional...Out All of Our Hot Plasmids Articles Browse the Repository  ...

...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

...co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...

...have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working with ...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving...

...studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an Alzheimer..., the experimental group, with the new kinase inhibitor and a second group, the positive control group...protocol but does not receive either protein kinase inhibitor. Since the negative control group does not receive...If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better to treat...available materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit ...

...and viral vectors containing genetic elements from rabies plasmids to international requestors. We also...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other ...

...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...