... AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot ...AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional...Out All of Our Hot Plasmids Articles Browse the Repository  ...

...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

...co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...

...have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working with ...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving...

...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844 . https... are even more exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of...breaks (Rees et al., 2018). For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window... guest blogger John Doench! John Doench is the Director of R&D in the Genetic Perturbation Platform at...

...studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an Alzheimer..., the experimental group, with the new kinase inhibitor and a second group, the positive control group...protocol but does not receive either protein kinase inhibitor. Since the negative control group does not receive...If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better to treat...available materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit ...

...and viral vectors containing genetic elements from rabies plasmids to international requestors. We also...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other ...

...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...

...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one ... the scientists who deposited plasmids to the repository and for the scientific sharing community (check... in 2016 and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving...

...? 11:51 - 13:28 : How do you define what a good mentor is? 13:29 - 14:31 : What drives you to do science...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentorship... mentor...

...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...reliably detect single-base differences. The original creators of this technique visualized the ligation product...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...responsible for guiding new deposits into the repository, performing quality control, and providing technical...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

...your community, think hard about picking a good mentor, and begin considering career options early. Tune...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentor...

... the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists find the plasmids...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...

...editing (SECURE) base editors Keith Joung’s lab recently found that a cytosine base editor (CBE) with rat APOBEC1... tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9 with gRNA ...

...resources. In 2022 alone, our website saw 2.2 million visitors from 231 countries. Guides and protocols were ...beloved blog received 1.3 million page views from visitors in over 200 countries. We, like everyone around...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science ...in the Netherlands. There are many academic administrators who are finding ways to provide new types of...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite (This is a...

...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early...

... Best Way to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris...posts on COVID-19 featuring plasmid tools from depositors who quickly shared their reagents: detection ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...

...   Alexa Fluor 700APC-H7APC-Cy7   Many factors can influence the level of brightness beyond the...detected through a cell surface label. The viral vector also encodes for eGFP, which serves as a transduction...example, point at issues with the double-expression vector.) In our flow experiment, we'll want to identify.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... Guide Ready-to-use antibodies in the Addgene repository Antibody Protocols ...