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Showing: 521 - 540 of 705 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...

  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

  6. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...

  9. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...theory you could use densitometric analysis to quantitate the intensity of the bands on your gel and get...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed...

  11. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...lane 1 is‌ lane 8. Common ways to keep membrane orientation straight are to mark the upper left-hand corner... Ensuring wash is happening under sufficient agitation (rocking) Add Tween-20 to wash buffer (can...

  14. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry...

  15. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ...

  16. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...be invited to compete in our speed round data presentation, with two winner each receiving a $500 travel...

  17. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...

  19. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...Addgene shipping and ordering processes, don’t hesitate to contact our friendly customer support team ...
Showing: 521 - 540 of 705 results