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We narrowed to 723 results for: Tat

Showing: 521 - 540 of 723 results
  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...
  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...
  3. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...
  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...
  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...
  6. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...definitely glad we did - you can see some of the stats from the AMA to the right - the Reddit community...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...
  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...
  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...
  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...
  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...
  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...
  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...
  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...
  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...
  15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...AAV receptor (AAVR)2 Laminin receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD).... (2006). Α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated...
  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...
Showing: 521 - 540 of 723 results