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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 719 results for: Tat

Showing: 521 - 540 of 719 results

  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

  3. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...

  5. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...

  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...

  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...

  15. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...AAV receptor (AAVR)2 Laminin receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD).... (2006). Α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...

  18. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...theory you could use densitometric analysis to quantitate the intensity of the bands on your gel and get...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed...
Showing: 521 - 540 of 719 results