We narrowed to 629 results for: UTY
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TypeBlog PostPublishedJuly 25, 2023, 1 p.m....Lentiviruses have gone through their fair share of lab evolution. There are 1st, 2nd, and 3rd generation plasmids...
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Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....Leemhuis, H., Kelly, R.M., Dijkhuizen, L. Directed evolution of enzymes: Library screening strategies. IUBMB... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....biotechnology.” These are the types of inquiries that routinely hit the inbox at the BioBuilder Educational Foundation... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....hybridomas will survive this step. Then, a limiting dilution is performed to isolate individual hybridomas ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....activated upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....the fluorescent protein properties need to be scrutinized under relevant conditions. The properties of ... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....luminescence to get around these issues, but this solution wasn’t practical due to the low intensity of luminescent... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m.... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....Goal setting is easier with more perspectives contributing to the process Senior people (of which there... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....their advice and time. Consider this option but be cautious and make sure to choose one that comes highly ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a technology that was difficult... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... many implications for regenerative medicine, evolution of body plans, synthetic morphology, and even ... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....information available. When I am speaking at a new institution, for example, I will often see a whole bunch ... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....fluorescent markers to visualize localization and distribution of gut bacteria without fixation or preparation... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series of... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....microenvironments shape a tumor’s evasiveness, evolution, and response to treatment. Some microenvironment... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....high-throughput proteins engineering methods, like directed evolution, because channelrhodopsins are typically evaluated...