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We narrowed to 911 results for: addgene

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Showing: 521 - 540 of 911 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... currently an Outreach Data Curation Intern at Addgene. She is also a graduate student at the University...PMCID: PMC3207894. Additional Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...instructions on how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used...natural resistances.  Additional Resources on the Addgene Blog Read other Plasmids 101 Posts Learn How to...Resistance on Our Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

  4. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...Bassik CRISPR Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale...Central PMCID: 3652613. Additional Resources on the Addgene Blog Learn to conduct genome-wide screens with...CRISPR Pooled Libraries currently available from Addgene Other Resources Watch Dr. Michael Bassik’s lecture...Guide to All Things Lentivirus Resources on Addgene.org Check out this guide to pooled plasmid libraries...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility...

  6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...share the plasmids created under this programs via Addgene. New organisms for genotype to phenotype studies...minute) talks so we got to hear from everyone. Addgene is certainly going to have to find ways to tag ...lab? Let us know! Additional resources on the Addgene blog Check out our other model organism blog posts...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version... chain and light chain are also available from Addgene!   Figure 1: Experimental validation for...

  9. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...training in a variety of transferable skills. At Addgene we're helping to fill this gap by both helping ... had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone...a-brief-exercise-to-spur-innovation-on-your-team   Additional Resources on the Addgene Blog Transferable Skills Guide: Problem-Solving...from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for example) and let your...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Plasmids for each of these systems are available from Addgene. If you’ve given any of these systems a try, we...acssynbio.0c00073   Additional resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

  12. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

  13. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

  15. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...need to get there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How ...

  16. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...perfect application. Additional Resources on the Addgene Blog Learn How to Experiment with New Careers ...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations...github @tlambert03.   Additional Resources on the Addgene Blog Fluorescence Microscopy Techniques - Which...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...plasmids from this publication at Addgene! Additional Resources on the Addgene Blog Find planarian tools for...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...understand how therapeutic strategies work. Find Addgene Blog posts about other educational tools Playing...twitter @FRE_SCI.   Additional Resources on the Addgene Blog Find planarian tools for teaching developmental...Arabidopsis Biological Resource Center Resources on Addgene.org Find molecular biology educational resources...
Showing: 521 - 540 of 911 results