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We narrowed to 664 results for: cell

Showing: 521 - 540 of 664 results

  1. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system will...US, I very much enjoyed interactions with many excellent colleagues at NYU and visiting scientists, such...In addition, I've been very lucky to work with excellent colleagues in my lab. On the other hand, I feel...

  2. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...useful for both in vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter...

  3. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...Studies Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...2016 Click on the links below to read all of our excellent posts from July: Addgene at the ASM Microbe 2016...

  4. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

  5. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh...developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher...biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's plans for...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

  9. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... this, you can increase the volume of competent cells you plate after each transformation and/or use different...recommend transforming into chemically competent cells in a 96-well PCR plate. Always confirm your results...

  12. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

  13. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

  16. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology and scientific...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...
Showing: 521 - 540 of 664 results