We narrowed to 800 results for: cha;
-
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled...
-
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) ...within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....general comments and adjust them if my opinion changed after this more detailed look. Encourage... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8 Chakiath CS, Esposito D (2007) Improved recombinational... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m.... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer...