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...detection Feng Zhang’s lab recently developed a platform called SHERLOCK (specific high-sensitivity enzymatic...plants, it has been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared...

... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...offered to answer readers' questions. If you have information or another resource to add, please tell us about...

...with biorender.com   Both gradients can be informative, so spend some time thinking about which type...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based...rule out) blocking issues.   If your antibody information doesn’t contain blocker recommendations, you ...

...scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines (WCAG)...

...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

...px or 1080 px).         Do you do a lot of transformations with the plasmids you receive from Addgene?...shipped as bacterial stabs? You can learn about what format your material will come as (and more!) on Addgene...

...dynamically.” To understand how that works in memory formation or other brain functions, Konermann needed tools...For those who do want to apply LITEs for the transformation of neurons, she offers a tip: save yourself...

...interests and skillset. With this information in hand, you can then formulate a plan to further develop your...

...inversions [3]. These rearranged segments may also be informative as to genomic function. A second use of targeted... Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts   ...

...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

...has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

...taking info from the code of viruses to give information to the computer to fight the known viruses. Bacteria.... As Dr. Mello said, “Cells have been in the information age for billions of years (…) Bacteria are really...

...of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein...fluorescent proteins are concerned.” For more information on choosing a backbone and making fusions, see...

...scientific outreach and community engagement. Information about contacting Addgene can be found on our ...struggled to get those plasmids with the right information and I believe that a lot of European students...

...vector containing the newly inserted gene is transformed into bacterial cells and selectively grown on...Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid...

...fluid (Darvesh et al., 2011). This technique is performed with a probe surrounded by a semipermeable membrane...The advantage of this method is that it can be performed in a live awake animal by surgical implantation...

...react by performing a distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous...

... they could solve this problem. D10A nickase outperformed both WT Cas9 sites tested by a wide margin, ...use Cas9 nickases in genome editing. For more information and a link to the archived webinar, click here...

...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

...the Rawlins Lab have compiled a new toolkit for performing genetics studies in organoids. The Organoid EasyTag...petunia SL enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the ...