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Showing: 521 - 540 of 766 results

  1. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...offered to answer readers' questions. If you have information or another resource to add, please tell us about...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...with biorender.com   Both gradients can be informative, so spend some time thinking about which type...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based...rule out) blocking issues.   If your antibody information doesn’t contain blocker recommendations, you ...

  3. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

  4. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines (WCAG)...

  5. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...px or 1080 px).         Do you do a lot of transformations with the plasmids you receive from Addgene?...shipped as bacterial stabs? You can learn about what format your material will come as (and more!) on Addgene...

  6. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...dynamically.” To understand how that works in memory formation or other brain functions, Konermann needed tools...For those who do want to apply LITEs for the transformation of neurons, she offers a tip: save yourself...

  9. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... bloggers. Check out this blog post for more information. Additional resources on the Addgene blog ...

  13. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...scientific outreach and community engagement. Information about contacting Addgene can be found on our ...struggled to get those plasmids with the right information and I believe that a lot of European students...

  14. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...fluid (Darvesh et al., 2011). This technique is performed with a probe surrounded by a semipermeable membrane...The advantage of this method is that it can be performed in a live awake animal by surgical implantation...

  16. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... they could solve this problem. D10A nickase outperformed both WT Cas9 sites tested by a wide margin, ...use Cas9 nickases in genome editing. For more information and a link to the archived webinar, click here...

  17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

  18. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

  20. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...vector containing the newly inserted gene is transformed into bacterial cells and selectively grown on...Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid...
Showing: 521 - 540 of 766 results