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We narrowed to 833 results for: ins

Showing: 521 - 540 of 833 results

  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... to train our young scientists for futures both inside and outside of academic research (4). There are...industry, regulatory, or clinical environment. It remains contested, however, whether a better solution would...University, has recently published an article arguing against reducing the number of graduate students accepted...grassroots organisations that represent postdocs and institutes that employ them, the diversity of trainee backgrounds...

  2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...though you may need to be on a network with an institutional subscription), archived on PubMed Central, or...Check out the hashtag #365papers on Twitter for inspiration and accountability. There are a few different...reprints and papers with scribbled notations in the margins. If you prefer, printing and hand-writing notes...guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences at Louisiana...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...number of important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance... Cre acts independently of any other accessory proteins or co-factors, thus allowing for broad applications...no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate locations in a DNA sequence... a parallel orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break ...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...that treat disease at the molecular level. For instance, if a disease is caused by a mutation in a single...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...showed significant clinical efficacy against a form of blindness known as Leber’s congenital amurosis ...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...

  5. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...high titer virus for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest... This is due to inefficient packaging of large inserts; a study by Kumar et al. found that in some cases...titers can drop 10-fold for every 2kb increase in insert size. To overcome this, lentivirus can be concentrated...require an ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...mostly uses whole-mount IHC techniques on rodent brains and is expanding to more complex brain systems ...larger-volume antibodies for thicker tissues and whole brains, we chose monoclonal antibodies (mAbs) and their...negative control which does not have primary and only contains secondary antibodies, allowing us to only image...

  7. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...). Figure 3: pegRNAs whose 3′ extension begins with a C base can cause unwanted base pairing (...BioRender.com. What to edit The sequence you choose to install can influence the success of your prime edit. If...sgRNA is designed to bind only after the edit is installed. This reduces concurrent nicks, which lowers indel...so that nicking only occurs after the edit is installed on the opposite strand. Created with BioRender.com...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin... changes with Ca while another wavelength is insensitive. Additional red sensors based on mRuby (jRCaMP1...Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services  Resources...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...in different fields that can provide different insight into an area - not just working within the established...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... applying for smaller grants from within your institute or a private funding body. Success breeds success...collaborators can also help you get some critical insight into what you're doing wrong. All this helps to...

  10. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...students and postdocs broadly – and thus PI’s and institutions in turn. The first is the passing and upcoming...the NIH recommended pay scale – used by many institutions to set postdoc wages. The second event of note...simply being able to categorize all postdocs at institutions, will be challenging. At the same time, there...level, FoR is currently compiling a database of institutional responses to the FLSA ruling. Similarly, from...

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), we developed...Perhaps most striking is that there were seven institutions involved in the effort. This inclusive and open...biomolecular tools can distinguish between global strains of the Zika virus (Asian-, African- and American-lineages...

  12. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...tail targets the RNA to the degradosome, which contains enzymes that cut RNA not protected by secondary...complex and involves two RNA polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting...Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal Polyadenylation...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus virus-like...VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions in the cell, improving...zCas9i cloning kit is a plant cloning kit that contains a series of vectors and DNA modules for assembly...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here are some tips...split cells Don’t be lazy about splitting cells, instead try to form a routine. Twice a week often works...take a vial of the ‘mint condition’ master stock instead of ‘borrowing’ cells that have already been passaged...soon be joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...helper plasmids or the cell genome can be packaged inside the vectors during production. These impurities...removed during the purification step because they are inside the virus itself. While these impurities are generally...NGS at Addgene to sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

  16. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...department during the application process. Many institutions recommend that you reach out to potential advisors...you can’t visit. By talking with people at the institution, you can get a better sense of the environment...answer process can also help you to see if the institution is right for you outside of just the program ...options are very handy in moments like that! For instance, during my application cycle, two schools on opposite...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...November 18, 2016): Researchers from a variety of institutions recently reported their inability to recapitulate... and development before it can truly be pitted against CRISPR head-to-head.  Exciting features of NgAgo...and fussy to optimize. One reason could be the instability of the supposedly alien 5’-phosphorylated ssDNA...Burgess, Shawn., et al. "Questions about NgAgo." Proteins & Cell (2016). 3. "NgAgo: Genome-editing by a ...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...labs of David Root and John Doench at the Broad Institute have developed human genome-wide CRISPRko (knock...CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations in the nuclease domain...potential off-target effects. The Dolcetto library contains two sets of sgRNAs; A, the top 3 ranked sgRNAs... sequences can also be modified to include RNA domains that recruit additional transcription factors. ...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...and that it often provides incredibly valuable insight into one’s work. As a community, we must therefore...remove the stigma around sharing negative data and instead focus on getting this information out into the ... A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A., Pereira, S....

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials could become our newest line of defense against bacteria. Created with BioRender.com. ...resistance using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged... just the method needed to jumpstart the fight against antibiotic resistance. Advantages of CRISPR over...strategy can help us win back ground in the fight against bacterial superbugs. References 1. Citorik, Robert...
Showing: 521 - 540 of 833 results