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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 714 results for: met

Showing: 521 - 540 of 714 results

  1. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...speaking, the PhD application process tends to go something like this:   However, as simply as it seems laid...program. An email to a potential PI should go something like this: Introduce yourself, brief educational...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...degron tags can offer over other protein control methods and compare the commonly available types of degron...protein post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate...

  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...these electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these... described systems. Several adaptations of this method have been to tag, cage, uncage, and trap the chemical...

  4. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...the circulatory or lymph systems to spread, or metastasize. Many cancerous cells will die trying, as the...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to fuse an ...jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...relationship is non-linear and you should use a different method of analysis. Different fields and assays have varying...

  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...barcoded tube (see next section) and every time something is removed from this tube, the barcode on the ...digit barcode and then assigned to a location in a metal freezer rack that contains five levels that can ...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... populations in drug screens, to visualize micrometastases in nude mice in cancer studies, act as a reporter...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...essential for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...with far fewer side effects compared to lesioning methods -  they may be the key to further unraveling the...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many... editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory of ...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...improves expression levels. Journal of Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(...base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels... of proximity labeling in living cells. Nature Methods, 20(6), 908–917. doi: 10.1038/s41592-023-01880-...

  19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...enzymes (BsaI, BbsI/BpiI, BsmBI), which recognize asymmetric DNA sequences and cleave at a short distance ...stocks beforehand. You can also use high-throughput methods to streamline your plasmid extractions. Use this...

  20. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...
Showing: 521 - 540 of 714 results