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We narrowed to 789 results for: mpl

Showing: 521 - 540 of 789 results

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...

  3. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two sites within the viral genome...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...

  5. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual...volunteered for various tasks as things came up. For example, I updated some sections of the NPA’s website. ...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...

  7. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...specific type of virus you are trying to make. For example, bacteriophages will need to be produced in bacteria... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...alternative PAM sequences are longer and often more complex than NGG, resulting in lower overall genome frequency... be less predictable than SpCas9 off-targets. Employing xCas9 for transcriptional regulation and base ...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...which multiplies every 16 hours). So if you, for example, split cells on a Monday diluting them 1:10, you...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...

  11. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...

  12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ... in proximity by the looping of chromatin, the complex of DNA and proteins that forms chromosomes. These... of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested into smaller...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ...

  13. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

  14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...happy to upload your newest resources. Here is an example of a plasmid with a downloadable protocol listed...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...

  16. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs, databases...consistently run our blastx searches with the “Low complexity regions” filter unchecked so that these regions...benefit is that blastp searches do not filter low complexity regions by default, meaning that you do not have...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small... a few time-tested tags and fusion proteins as examples. The tables list more common tags than are described...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... array of biological disciplines and scientists employ GFP for a wide number of functions, including: ...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...modified ES cells, which can then be used for implantation and generation of transgenic mice. Purification...

  20. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an... in the family to have to miss something – for example, say no to some of those birthday parties. Tell...time. For me, that’s been volunteer work at our temple, my kids’ school, and with professional organizations...
Showing: 521 - 540 of 789 results