We narrowed to 801 results for: omp
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TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling FRET measurements...
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CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m.... this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish transgenic.... Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox Hot Plasmid 2: AAV Vectors for tageting... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...1000 Lanes Cost $200-$500 <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between... -
Antibody Data Repositories and Search Engines
TypeBlog PostPublishedOct. 31, 2024, 1:15 p.m....experiment. Search engines let you easily find and compare available antibodies from many vendors, while data...Search engine May include validation data Biocompare Any Any Search engine Are we missing... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m.... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species. When he found the chromoprotein... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog PostPublishedOct. 5, 2017, 1:13 p.m....multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....decorated office! Below, they turned one of the lab computers into a fireplace scene. Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m....neutralization or targeted destruction. Antibodies are composed of two light chains and two heavy chains. The ...the use of less toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog PostPublishedApril 22, 2019, 12:26 p.m....lab equipment on? This applies to water baths, computers, and more. Consolidate your autoclave runs. In...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things... -
Make a Splash: Notions of Scientific Impact Are Evolving
TypeBlog PostPublishedDec. 5, 2014, 2:54 p.m....measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....about careers that we’ve compiled our top career blog posts into one comprehensive, downloadable guide: the... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m.... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...the detection of RNA, dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs ... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....gRNAs to knockout genes – are essential screening components, the model system and the assay are just as critical...him that he has the best words and fingers of a completely normal size. References: 1. Doench, John... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...