We narrowed to 599 results for: REV;
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TypeBlog PostPublishedOct. 9, 2014, 3 p.m....brightness, photostability, and so on; see the previous blog post for more discussion of these factors...that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent...
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From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....focus on my core responsibilities, which typically revolved around the broader vision rather than the day-...mistakes and learn from them. I started at my previous company by taking on responsibilities such as ... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....allowing for the targeting of sites that were previously unavailable. Figure 1: The PAM sequence...specific location, it must be accessible by Cas9. Our previous blog post The PAM Requirement and Expanding CRISPR... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development... the morning! 9:00 AM--Technical implementation review. The developers discuss which front-end and back-end... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m.... other fields or writing grant proposals to be reviewed by interdisciplinary panels. And highly successful...Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online with... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m....collaborator Some of these people you meet will review your papers or grants in the future, and don’t ... are talking to is really in your direct area. Review the conference schedule and attendance list. Plan...