...biological sciences and constitute foundational elements of biomedical research. Traditional restriction...purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning...directly into chemically competent E. coli and transformants selected with the appropriate antibiotic. Although...

...attractive imaging platform for in vivo imaging experiments because  ultrasound devices are inexpensive, ...coli strain Nissle 197 (ECN) was used for these experiments because it’s a probiotic strain that can colonize... and inflammatory bowel conditions. In these experiments, arg1 expressing ECN were injected into the mouse...

... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

... A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies: A database... not listed in today’s post? Share them in the comments below. This post was written in April 2020 by ...protocols using apps to save you time and track your experiments  Resources on Addgene.org: Read our Molecular...

... use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing...two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than HcKCR2, making...long-sought tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer...

... variety in their job. Good relationships with clients and colleagues Sometimes this means you have to... in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be the...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...

...in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...

...find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy?  Contact...

...RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains...

...reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that...

...hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ...

... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

...of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and...

...measured through their triglyceride or cholesterol contents (what is commonly called bad cholesterol). However... and localization in vivo. The LipoGlo system represents a new, intriguing approach to expand our understanding... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

...Once you’ve identified some fly stocks and other reagents of interest, the next question to ask is what ...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Although it’s often very easy to get GAL4 UAS experiments up and running with fly lines that are already...

...Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years studying...: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration; Laurenz Studer...focused on translational research is encouraging. Patients are key to the research, but they are also the...

...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found...classical purification protocol involving iodixanol gradients. The current purification protocols for isolating...decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs...

...high-security facility needed to perform the experiments to demonstrate that this approach was useful ...diverse. Some of them are undergraduates or PhD students who are just starting their journey into science...stops these one-time favors from becoming starting points for long-lasting partnerships Join us! We are a...

...candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods...