We narrowed to 803 results for: TIM
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TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....scientists of all areas who volunteered to donate their time and expertise to help them. In less than two weeks...and easily credited. And nothing stops these one-time favors from becoming starting points for long-lasting...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical... and conditions that can be used to monitor real time voltage changes in worms and have made these constructs... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....bogged down with distributing them and spending more time on research. What do you hope Addgene will accomplish...and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. Additional... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m.... and develop skill in science communication over time. Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or... Berkley Gryder. Addgene depositors or not, your time is valuable ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....transgenic flies to study your favorite gene (YFG). Sometimes, you want to use the Gal4/UAS system, but the ... as the intestines, can be dissected at various times throughout the lifespan of the fly to determine ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....perform under your experimental conditions. This is time well spent and, if you do it right, can be (part...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m.... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....software tools for the right people at the right time. To explain a bit further, a software product manager...going to need to be a problem-solver. A lot of the time, the problem to solve is clear, but the solution... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased editing...the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution of naturally ... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” or novel for their... understand the difference between the two, it’s time to check out our CRISPR library resources or our... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....catalog are very well-characterized. During this time, we’ve also created a variety of educational resources...the foundations for many improvements to come. Ultimately, we would like our search engine to be so specialized... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m.... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest away the...-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works in your... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...