We narrowed to 845 results for: CAL;
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TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...
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Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....from the Neuroscience Information Framework (NIF) called the Resource Identification Initiative. During ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....With our protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....too long in the incubator. The small colonies are called satellite colonies and they form when all the antibiotic... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....dosage in E. coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... targeting the Fc region of an antibody, the so-called ‘constant’ region which is, quite usefully, both... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m.... the desired level of antibodies is reached - typically within 2-4 months. After the animal has the desired... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m.... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....cytoplasm or pronucleus of the zygote which is both technically challenging and can negatively impact zygote ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....Biodesign institute developed a 3’UTR database specifically for C. elegans. This database provides detailed... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....-humanized (Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....and Change in Undergraduate Biology Education: A Call to Action, Washington, DC. 2. President's Council... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....s outer membrane and allows the components to physically enter the cells so they can go to work. ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m.... not schematized. Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m....: my undergrads were different people! More specifically, they had different learning styles. One was... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....2,500 protocols covering a variety of bio and biomedical topics. Protocol Online Website: http://www.protocol-online.org... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... useful also. There is also a U.K. organization called Athena SWAN that is a charter for advancing women... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....plants: Lots of plants produce lots of seeds. I typically use 8-10 plants per construct I transform. More... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....Updated Aug 13, 2020. Note: Cpf1 is also called Cas12a. In 2015, Zetsche et al. added to the CRISPR ... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....community is supply chain shortages. When flasks, conical tubes or pipette tips are backordered for months... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the research of Drs. Feng...