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  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...better interpretation. Priyamvada Prathima has a research background in protein mutagenesis and cancer immunotherapies... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School...

  3. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

  4. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...Coming from a strong background of undergraduate research straight to a lab career at a biotech company,...final report. Work on a final report for a user research project I led. It’s divided into the key takeaway...

  5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...the many cells that don’t. One way to begin the search is by using selection strategies, where only cells...can hydrolyse lactose. Fortunately, for the lab researcher, when the substrate X-gal is broken down by 𝛽-galactosidase...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... of a biosensor Some of the biosensors used in research are very sophisticated, but at their core they...example, calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...features with gamma-retrovirus including genome architecture, host integration, and its association with ...reviewed the common viruses used in biological research, you might have an idea which ones could be helpful...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...expression, or poor scalability.  Scientists at Janelia Research Campus and the University of California, Berkeley...AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  9. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... cell. Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough...are four main types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV genome design...Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... antibody in the lab, you’ll therefore want to research your conjugation reaction to understand if any... an experiment with many proteins of interest! Research the brightness of your fluorophores before buying...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...improve the utility of CRISPR/Cas9 in new avenues of research. The use of multiple gRNAs targeting a single ...editing. Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression...

  14. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...attendance list. Plan the sessions you will attend. Research the speakers in advance and make a note of those...and promotions.  Follow academic scientists on ResearchGate and/or via Pubmed alerts. Congratulate your ...

  15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style.... Alumni who enter their information in these searchable databases are usually offering their contact ...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN kit to quickly...spoke to Musunuru about how the new kit works, the research the kit now makes possible, and how it compares...

  17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...greatest’ variants with the best properties for your research. But be aware that the fluorescent proteins reported...under the conditions that are relevant for your research. This will reveal the performance of fluorescent...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...coli biotin ligase BirA* from BioID and allows a researcher to identify proteins that bind an RNA motif of...biotinylated background proteins reported by other research groups that performed BirA*-based affinity purification...

  19. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...without disrupting protein function. A literature search can help determine which terminus is favorable ...the ENL YEATS domain in acute leukemia. Nature. March 9 2017. 543(7644): 270-274. PubMed PMID: 28241139...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...former Caltech researcher, now Director at the Max Planck Institute for Brain Research in Frankfurt am...
Showing: 541 - 560 of 795 results