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We narrowed to 898 results for: CAN

Showing: 541 - 560 of 898 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...internal region of the mRNA. In the figure above, you can see that the IRES element acts as another ribosome... L L K L A G D V E S N P G P * (GSG) residues can be added to the 5' end of the peptide to improve ... the co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites...PCR-cloned between your genes of interest and you can then insert the whole multicistronic cassette into... 101 eBook And now for some exciting news... You can now download Addgene's new eBook! Our goal was to...additional resources into one downloadable PDF that you can save on your desktop. The Plasmids 101 eBook is designed...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...well as their modern equivalents and see what we can learn from their evolution. Southern blotting and..., named for its inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance...has a particular gene mutation, southern blotting can be used to identify the presence of that mutated ...bind to the sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film...are also commonly used. In all cases, the result can be quantified by the strength of the signal in comparison...for cloning and analytical digests. A researcher can test the orientation of an insert in only a few hours...nucleotide, a researcher (or a simple computer program) can then reproduce the DNA sequence (Figure 2). Accuracy...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...A viral vector that can target specific tissues, even when administered systemically, without causing...Recombinant adeno-associated viral vectors, or rAAVs, can sound almost too good to be true! In a previous post...genetic material, and variations between serotypes can change how the viral particle sneaks its way into...multiple tissues, rapid transgene expression, and can cross blood vessel barriers. It is especially popular... targeting cardiac cells (Bish et al., 2008). It can also cross the blood-brain barrier, making it useful...receptor published this year (Dhungel et al., 2025)! It can transduce neurons for retrograde labeling, as well...addition to tissue tropism, different AAV serotypes can be useful to evade neutralizing antibodies developed...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...kinds of problems can, of course, be solved with good management practices, but can sometimes go unnoticed...those contracts fall through or are cancelled, then redundancies can be made within the company; new staff...large company (both for-profit and non-profit) you can also get a lot of office politics (many individuals...projects and away from other individuals projects, this can cause conflicts and favoritism, as well as affect...have less work to do than was anticipated and there can be a glut of individuals with similar skill sets....was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia or... my job. Because of my background in genetics, I can help scientists problem solve experiments over the...

  5. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...more about their work, and how these plasmids can help cancer researchers studying an assortment of genes...population, as mutations with strong phenotypic effects can mask weaker ones. Thus, the need for a faster and...phenotypic effect in a different system. These plasmids can also help scientists understand how a given gene ... genetic perturbations that may play significant roles in cancer pathogenesis. Their work culminated ... from The Cancer Genome Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics...interventions for cancer. This screening platform is an incredible boost to the cancer research community...functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): 450-462. PubMed PMID: 29533785...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ..., high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm past the surface...CRISPR system. This common optogenetic technique can be employed in other split systems where light is...2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous end joining ...Single-chain Cas9 photoswitches Photoactivation of Cas9 can also be achieved using single-chain fusion proteins... of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was...Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific genomic loci for imaging...imaging techniques. Since expression of dCas9 can potentially alter the genome and chromatin architecture...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ... role in the severity of breast cancer. Infectious Disease. RaPID can also help identify which host proteins...naturally occurring complexes present in the cell, it can also introduce non-physiological RNA-protein interactions...like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...to Zika replication. Human genetic disease. RaPID can also be used to test how RNA variants associated ...patients. These are just three examples of how RaPID can be used to study RNA-protein interactions. Do you...in breast cancer patients. By digging into the literature and analyzing data from The Cancer Genome Atlas...other BirA*-based proximity labeling methods, RaPID cannot distinguish between proteins that directly or indirectly...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cpf1 can process its own pre-crRNA arrays. Zetsche et al demonstrate this by showing that Cpf1 can cleave... to express the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences...is a suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging...repeats, leading to expression of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was ...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then be used...learn more about it? Read on to find out how ccdB can make your cloning experiments a little easier. One...ccdB alone isn’t the only key to the system – how can one work with a plasmid/gene that kills the cells...function, there is some evidence that ccdB Survival™ can be more difficult to transform, depending upon the...

  12. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...used for targeting; however, these options can be cumbersome, can lower on-target efficiency, and sometimes...similar mutations can improve the specificity of Cas9 derived from S. aureus. This means you can likely apply...It is well known that CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means...

  13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    .... Thus, T-DDOGs can be used in combination with green-red Cre-lox systems. T-DDOGs can also be designed...T-DOGGs can be made drug-inducible using the rTetR system, but Cre-DOG lacks the toxicity that can be seen...their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly to GFP...

  14. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target editing. In this...both strands bluntly with WT Cas9 and one gRNA, you can create a staggered cut using a Cas9 nickase and two...target different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation...20% repair efficiency. Clearly, a nickase strategy can expand the targetable region, which is especially...site. So if you can’t find a good gRNA that cuts close to your insertion site, you can’t obtain high HDR...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...had success with significantly fewer wings, such that enough tissue for an experiment can be easily collected...obtaining enough material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  ...short incubation at 37 °C. These short DNA fragments can then be purified for subsequent library preparation...science.     Additional Resources Learn how you can use FRET to study protein interactions Learn about...Washington. CUT&RUN doesn’t require fixation and significantly decreases background noise, reducing the read...improvements to the initial CUT&RUN protocol, the most significant being the usage of a strong detergent to permeabilize...extraction. They report that this change offers a significant improvement and simplification of the original...

  16. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...responsibility in our lab’s environmental impact, but we can all do more to make research more environmentally...will likely end up in a landfill, but at least we can reduce the rate at which that happens and reduce ...t offer plastic recycling, specialized companies can recycle plastic-- you just have to find them (or ...commonly accepted at recycling facilities. Your lab can buy a box with the prepaid label and fill it with...

  17. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...Rosella can be used to track the targeted autophagy of mitochondria, or mitophagy. Rosella can also be...autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk autophagy, but the addition...degradation by autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a C-terminal glycine...fluorescent relies on lysosomal acidity, this biosensor can’t be used with fixed cells. Find the mitochondria-targeted...

  18. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...scientists with new tools and protocols that they can easily incorporate into their own labs. This week...submission of the results of our first study which can be accessed here at bioRxiv. AAV2 interacts with ...that this effect is not specific to sucrose, but can be adapted to other sugars such as sorbitol. As sorbitol...excited? For starters, we believe that this approach can be adapted by vector cores and laboratories alike...viral particle’s affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe...

  19. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...more precisely control the amount of labeling, you can alter the attP site. Truncating the attP site upstream...Applications for SPARC SPARC technology is versatile and can be applied across cell types and organisms by replacing... the construct. In Drosophila, the SPARC systems can be used both for visualization and for optogenetics...of fly cell types. Because the PhiC31 recombinase can work in other model organisms including mouse and...

  20. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...plasmids articles. Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral...Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form...tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer. The current... is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of interest and...
Showing: 541 - 560 of 898 results