We narrowed to 568 results for: CHI

1. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

2. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy...

3. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

4. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...your particular setup. We recommend starting by searching the literature to see what is already known about...

5. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised...

7. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ... or blog on optimizing this step, or consider enriching for transfected cells by flow sorting or drug ...

8. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...

9. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...

10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding...

11. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ... introduce a frame-shift mutation. This can be achieved using a restriction enzyme that recognizes a unique...

12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...hurdles. The first is the delivery of the CRISPR machinery to all of the target cells. This goal may prove...

13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...editing faces the challenges above and more. One chief challenge is upregulating HDR in mature tissues,...

14. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count the number ...

15. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

16. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...

17. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds...

18. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...ticker tape” method for intracellular recordings, switching between different colored ligands at different...

19. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ... it’s important to know what you are aiming to achieve. In general, these are three core goals of antibody...

20. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...target site (Fig. 3B). The INTEGRATE system can achieve ~100% integration of DNA up to 10 kB in size in...