We narrowed to 571 results for: CHI

1. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...

2. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...benefit when recruiting patients, specifically children and patients with skin disorders for research ...

4. Career Insights: Technical Support Specialist

   Type

   Blog Post

   Published

   Nov. 9, 2017, 2 p.m.

   ...rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This...

5. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...mycoplasma can be extremely hard to detect with levels reaching 108 cells per ml before the media becomes cloudy...

6. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

7. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...your particular setup. We recommend starting by searching the literature to see what is already known about...

8. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

9. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...

10. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ... or blog on optimizing this step, or consider enriching for transfected cells by flow sorting or drug ...

11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type

    Blog Post

    Published

    Sept. 28, 2023, 1:15 p.m.

    ... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised...

12. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...

13. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding...

14. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ... introduce a frame-shift mutation. This can be achieved using a restriction enzyme that recognizes a unique...

15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...hurdles. The first is the delivery of the CRISPR machinery to all of the target cells. This goal may prove...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...editing faces the challenges above and more. One chief challenge is upregulating HDR in mature tissues,...

17. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count the number ...

18. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...

19. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds...

20. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...