We narrowed to 678 results for: Eng

1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of...

2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

   Type

   Blog Post

   Published

   Sept. 17, 2020, 1:15 p.m.

   ...difficult time. One function that rose to the challenge was our Finance Team. With Addgene closed for ...

3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   Published

   Aug. 11, 2020, 1:15 p.m.

   ...sequencing conditions which can read through this challenging sequence. Transgene The transgene is the gene...

4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

   Type

   Blog Post

   Published

   Aug. 8, 2019, 1:19 p.m.

   ...authors put in the manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge...

5. New and Upcoming Viral Vectors - Spring 2019

   Type

   Blog Post

   Published

   March 26, 2019, 1:40 p.m.

   ... AAV The GFP-based calcium sensors are single-wavelength sensors with intensities that change in response...

6. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   Published

   Jan. 25, 2018, 2:09 p.m.

   ...interest fused to maltose binding protein (MBP, New England BioLabs). MBP has a similar function as GST in ...

7. What Do I Do Now? Academic v. Non-Academic Career Decisions

   Type

   Blog Post

   Published

   March 9, 2017, 3:40 p.m.

   ...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...

9. Antibodies 101: Epitope Tags

   Type

   Blog Post

   Published

   May 11, 2023, 1:15 p.m.

   ...doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the need for target-specific...

10. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...stable frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672...

12. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone ...

13. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2015, 2:30 p.m.

    ...edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content...

14. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...patients. Waardenburg Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol University’s Ramathibodi...

15. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    Published

    Aug. 25, 2015, 2:30 p.m.

    ..., including one led by a talented biomedical engineering team from MIT.   I would like to share my own...

16. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    Published

    Aug. 21, 2014, 3:15 p.m.

    ...was researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most...

17. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    Published

    June 18, 2019, 1:15 p.m.

    ...lab recently deposited PSAM AAV, which encode engineered ligand-gated ion channels that can be activated...

18. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nature Biotechnology...

19. The MTA at Addgene

    Type

    Blog Post

    Published

    July 23, 2024, 2:17 p.m.

    ...materials, and the process was time-consuming and lengthy. So the NIH called a meeting in 1990 to discuss...

20. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and...