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We narrowed to 889 results for: IND

Showing: 541 - 560 of 889 results

  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...two most popular plasmids of the past 10 years. Induced Pluripotent Stem (iPS) Cells - 2008 was marked ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

  2. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...methyltetrahydrofolate, which are universally abundant. Second, induction of transcription occurs within minutes of light...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more...proteins (proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to...

  4. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...mechanism. The Cas13 effector binds the guide RNA (shown as mature crRNA) and binds the RNA target before initiating...often recruits the main effector complex after binding to a target. Class 1 is the most abundant of the... functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment by Cascade... large-scale clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    .... Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein resources...the practicalities and considerations you may not find in the materials and methods sections of optogenetics...Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nature neuroscience. ...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...determine if there have been any early successes, and to find out what delivery techniques researchers are using...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...programming noob (like myself) can understand. You’ll also find actionable advice on how to give a great science... (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order here...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which particular...Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled Libraries for Your Research...

  8. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

  9. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

  10. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...testing entirely new gRNA designs. You can now easily find many validated gRNAs in our newly curated Validated...search for ‘sapiens activate’ to filter the table and find exactly what you're looking for. Search using the...alternate names, particularly for gene names. If you find a sequence that will work for your experiment, it...Browse gRNA Design Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...

  11. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...

  12. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers...easy to miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela... look the same in every lab, but the principles behind this technique are beneficial for researchers at...   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...

  13. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... Below Bauer and Canver discuss the motivations behind this research.   Using CRISPR/Cas9 for targeted...deletion, the nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the...from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank you to our guest... mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian...

  14. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience.... During transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction... the AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV ...our AAV guide Learn more about our viral service Find retrograde AAVs at Addgene ...

  15. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...about how I could fill this time. I was relieved to find out that the two hours were split up between several... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...at Addgene. This training prepared me for the independent work on the QC team. I was responsible for helping...Addgene that means that it is possible to work the remainder of the three hours (outside of the core hours)...

  16. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems...annual NIH progress reports information on the Individual Development Plans (IDPs) for the graduate students...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

  17. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

  18. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...companies can recycle plastic-- you just have to find them (or keep reading). Corning takes back and recycles...take to action! These days, it’s important for individuals, companies, and universities to be sustainability... the Addgene blog Subscribe to the Addgene blog Find lab tips for your research Read another blog post...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

  19. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone and...for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava...supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor (Right),...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase...more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional...the power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18...
Showing: 541 - 560 of 889 results