We narrowed to 776 results for: IRE

1. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

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   Jan. 30, 2020, 2:15 p.m.

   ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

4. Viral Vectors 101: Inverted Terminal Repeats

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   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...usually an issue as they are thought to be self-repaired during the AAV replication process (Chen et al...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

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   Jan. 21, 2025, 2:15 p.m.

   ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...

7. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

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   July 28, 2017, 1:30 p.m.

   ...same chromosome. Targeted Insertion allows for direct comparisons between lines expressing different ...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...

8. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ... contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of...Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer Program at the Broad Institute of...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

11. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

12. Summer SciComm: Preprints

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    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

13. Antibodies 101: Affinity Reagents

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    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

14. Transferable Skills Guide: Problem Solving

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    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...BioRender.com. Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...

16. Click Biology: How to Click and Play to Explore Biology

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    Blog Post

    Published

    Oct. 21, 2025, 1:15 p.m.

    ...Besides requiring stable covalent reactions, other required parameters of the coupling system include: Wide... or C-terminal PCV2 HUH-tag; increase homology-directed repair efficiency by linking the repair template...

17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals or modifications. This...optogenetic tools can be difficult because this requires detailed structural knowledge of the proteins....

18. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

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    Feb. 28, 2019, 1:34 p.m.

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...

19. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

20. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...peptide derived from cytochrome p450, CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...