We narrowed to 776 results for: IRE

1. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

3. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

4. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...

6. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...usually an issue as they are thought to be self-repaired during the AAV replication process (Chen et al...

7. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

11. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...peptide derived from cytochrome p450, CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

14. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals or modifications. This...optogenetic tools can be difficult because this requires detailed structural knowledge of the proteins....

16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...

17. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

18. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

19. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

20. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...BioRender.com. Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...