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Showing: 541 - 560 of 619 results

  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...

  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... NYN PAMs, but with less efficiency and less reliability. Notably, the SpRY variant didn’t display higher...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this method is...

  7. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...we use. Scientists utilize an array of tools that rely on DNA amplification and manipulation to produce...

  8. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...search and replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...containing FLEx technology. Read the genOway press release for more information. References: 1. Schnütgen ...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the oldest system reviewed here. The AID system relies on three components. First, the protein of interest...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...the self-labeling tags SNAPf and CLIPf. These tags rely on synthetic dye molecules, which are much brighter...
Showing: 541 - 560 of 619 results