We narrowed to 610 results for: SCR

1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ...

3. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...

4. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and...

5. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...

6. 6 Tips for Grant Writing

   Type

   Blog Post

   Published

   Oct. 18, 2016, 2:30 p.m.

   ...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...

7. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

8. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

9. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ...manual skills at the regular microscope, and the scripting skills at the automated microscope  to keep our...

10. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...whether or not newly synthesized or long-lived transcription factors control gross changes in gene expression...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...

14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...

15. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...double-stranded break, while modulators regulate transcription and chimeric efforts make a precise edit or ...

16. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

17. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

18. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

19. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...

20. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...