We narrowed to 613 results for: Sis;
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TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....backbones expressing blasticidin or puromycin resistance genes. It’s that simple! Good luck and happy... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m.... codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....Shah's and Jim Well's lab induces cre-dependent apoptosis. Now available as a ready to use viral prep in... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....positive selection marker such as an antibiotic resistance gene is also included to select for successfully... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of generating... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....proof-of-principal study were very helpful in assisting me with applications for much larger fellowships... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....access to rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....literature review, survived technical feasibility analysis, and was a marketable product. It was a small ... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m.... & Lopez-Berestein, G. (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue...