We narrowed to 806 results for: Tes
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TypeBlog PostPublishedMay 18, 2017, 2:30 p.m.... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment Detection Method Notes Quantification of a process Plate-based assays...
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Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!) Fig. 1: HeLa cells. Image courtesy of Josef Reischig. Contributing to Cellosaurus... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....pages, performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website: A yellow flame indicates that a plasmid has been requested 20+ times ... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m.... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be.... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Montana State University National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) National... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....KAUST, Saudi Arabia, in the group of Prof. Mark Tester, where she focuses on (1) salt-induced changes ...Credibility, peer review, and Nature, 1945–1990." Notes and Records: The Royal Society Journal of the History... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...through successive rounds of mutagenesis for the brightest teal fluorescent proteins that were photostable... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m.... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes. ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....fields of biology and enable the conversion of RNA templates to DNA but suffer from several important shortcomings...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated that a reasonable... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...repository Name Serotype Category Depositor Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....applied for identification of essential MYC binding sites in a wide range of human cells. A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons. New...